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Efficient lipofection of human trophoblast cells in primary cultures
Jacquemin, P.; Alsat, E.; Oury, Cécile et al.
1993In Biochemical and Biophysical Research Communications, 196 (1), p. 376-81
Peer reviewed
 

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Keywords :
Cells, Cultured; Enhancer Elements, Genetic/*genetics; Female; Gene Expression Regulation; Giant Cells; Humans; Placental Lactogen/biosynthesis/*genetics; Pregnancy; Pregnancy Trimester, Third; Recombinant Fusion Proteins/biosynthesis; Tissue Distribution; Transfection/*methods; Trophoblasts/cytology/*physiology
Abstract :
[en] Human choriosomatomammotropic hormone, also known as placental lactogen, is expressed in syncytiotrophoblast cells of the placenta. Studying transcriptional regulation of the genes coding for this hormone, we became interested in transfecting primary cultures of these trophoblast cells. In this study, we show that it is possible to transfect, by the lipofection method, these giant cells in an efficient and reproducible manner. We show the presence of an enhancer region downstream from the hCS-B gene, functionally active in these cells; furthermore, we demonstrate the placenta-specific characteristic of this enhancer, previously identified in a human choriocarcinoma cell line.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Jacquemin, P.
Alsat, E.
Oury, Cécile  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R : Génétique générale et humaine
Belayew, A.
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Evain-Brion, D.
Language :
English
Title :
Efficient lipofection of human trophoblast cells in primary cultures
Publication date :
1993
Journal title :
Biochemical and Biophysical Research Communications
ISSN :
0006-291X
eISSN :
1090-2104
Publisher :
Academic Press, San Diego, United States - California
Volume :
196
Issue :
1
Pages :
376-81
Peer reviewed :
Peer reviewed
Available on ORBi :
since 26 August 2009

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