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Thyrotropin-releasing hormone and epidermal growth factor induce human prolactin expression via identical multiple cis elements
Berwaer, Monique; Peers, Bernard; Nalda, Asuncion M et al.
1993In Molecular and Cellular Endocrinology, 92 (1), p. 1-7
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Keywords :
Animals; Binding Sites; Cyclic AMP/physiology; *DNA-Binding Proteins; Epidermal Growth Factor/*pharmacology; Humans; Pituitary Neoplasms; Prolactin/biosynthesis/*genetics; *Promoter Regions, Genetic; Rats; Recombinant Fusion Proteins/biosynthesis; *Regulatory Sequences, Nucleic Acid; Thymidine Kinase/genetics; Thyrotropin-Releasing Hormone/*pharmacology; Transcription Factor Pit-1; Transcription Factors/metabolism; Transfection; Tumor Cells, Cultured
Abstract :
[en] Pituitary GH3 cells were transfected with different deletion mutants of the human prolactin (hPRL) promoter fused to the CAT reporter gene. The proximal region (-250 to -42) was sufficient to confer stimulation by both thyrotropin-releasing hormone (TRH) and epidermal growth factor (EGF). Further deletion analyses demonstrated the importance of the three proximal Pit-1 binding sites in this response. However, Pit-1 binding oligonucleotides confer neither TRH nor EGF induction to a linked neutral promoter, suggesting that other elements might be involved. We have previously shown that sequence A (-115 to -85) is needed together with Pit-1 binding sites for full cyclic AMP response of hPRL-CAT. Mutation of this sequence strongly affects TRH and EGF induction. On the other hand, three copies of sequence A confer both TRH and EGF response to a linked neutral promoter. In conclusion, although TRH and EGF activate mostly different intracellular pathways, they mediate transcriptional induction of the hPRL promoter via identical cis elements.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Berwaer, Monique;  Université de Liège - ULiège
Peers, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Nalda, Asuncion M;  Université de Liège - ULiège
Monget, Philippe;  Université de Liège - ULiège
Davis, Julian R;  University of Manchester
Belayew, Alexandra;  Université de Liège - ULiège
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Thyrotropin-releasing hormone and epidermal growth factor induce human prolactin expression via identical multiple cis elements
Publication date :
1993
Journal title :
Molecular and Cellular Endocrinology
ISSN :
0303-7207
eISSN :
1872-8057
Publisher :
North Holland Publishing Company, Amsterdam, Netherlands
Volume :
92
Issue :
1
Pages :
1-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 August 2009

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