Reference : Thyrotropin-releasing hormone and epidermal growth factor induce human prolactin expr...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/19876
Thyrotropin-releasing hormone and epidermal growth factor induce human prolactin expression via identical multiple cis elements
English
Berwaer, Monique [Université de Liège - ULg > > > >]
Peers, Bernard mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Nalda, Asuncion M [Université de Liège - ULg > > > >]
Monget, Philippe [Université de Liège - ULg > > > >]
Davis, Julian R [University of Manchester > > > >]
Belayew, Alexandra [Université de Liège - ULg > > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
1993
Molecular & Cellular Endocrinology
North Holland Publishing Company
92
1
1-7
Yes (verified by ORBi)
0303-7207
Amsterdam
The Netherlands
[en] Animals ; Binding Sites ; Cyclic AMP/physiology ; *DNA-Binding Proteins ; Epidermal Growth Factor/*pharmacology ; Humans ; Pituitary Neoplasms ; Prolactin/biosynthesis/*genetics ; *Promoter Regions, Genetic ; Rats ; Recombinant Fusion Proteins/biosynthesis ; *Regulatory Sequences, Nucleic Acid ; Thymidine Kinase/genetics ; Thyrotropin-Releasing Hormone/*pharmacology ; Transcription Factor Pit-1 ; Transcription Factors/metabolism ; Transfection ; Tumor Cells, Cultured
[en] Pituitary GH3 cells were transfected with different deletion mutants of the human prolactin (hPRL) promoter fused to the CAT reporter gene. The proximal region (-250 to -42) was sufficient to confer stimulation by both thyrotropin-releasing hormone (TRH) and epidermal growth factor (EGF). Further deletion analyses demonstrated the importance of the three proximal Pit-1 binding sites in this response. However, Pit-1 binding oligonucleotides confer neither TRH nor EGF induction to a linked neutral promoter, suggesting that other elements might be involved. We have previously shown that sequence A (-115 to -85) is needed together with Pit-1 binding sites for full cyclic AMP response of hPRL-CAT. Mutation of this sequence strongly affects TRH and EGF induction. On the other hand, three copies of sequence A confer both TRH and EGF response to a linked neutral promoter. In conclusion, although TRH and EGF activate mostly different intracellular pathways, they mediate transcriptional induction of the hPRL promoter via identical cis elements.
http://hdl.handle.net/2268/19876
10.1016/0303-7207(93)90068-U
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3G-47NWF8V-23&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=1bf4d1ca18d8f60647f4c5bcc71ad180
1993/03/01

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