Reference : A recombinant viral haemorrhagic septicaemia virus glycoprotein expressed in insect c...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/19856
A recombinant viral haemorrhagic septicaemia virus glycoprotein expressed in insect cells induces protective immunity in rainbow trout
English
Lecocq-Xhonneux, F. [EUROGENTEC > > > >]
Thiry, M. [EUROGENTEC > > > >]
Dheur, I. [EUROGENTEC > > > >]
Rossius, M. [EUROGENTEC > > > >]
Vanderheijden, N. [EUROGENTEC > > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
de Kinkelin, P. [Institut National de la Recherche Agronomique France > > > >]
1994
Journal of General Virology (The)
Society for General Microbiology
75
1579-1587
Yes (verified by ORBi)
0022-1317
1465-2099
London
United Kingdom
[en] Viral ; infection ; vaccine
[en] Viral haemorrhagic septicaemia (VHS) is a fish rhabdovirus infection of world-wide importance. Control policies have been established but the disease still causes heavy losses in fish farming. The development of a recombinant subunit vaccine was initiated to produce a safe and effective vaccine to protect fish against VHS. The VHS virus (VHSV) glycoprotein, which induces neutralizing antibodies in rainbow trout, was chosen for expression in insect cells using a baculovirus vector. The Mr of the recombinant protein estimated by SDS-PAGE was slightly lower than that of the native viral protein. The recombinant protein displayed different degrees of glycosylation and was recognized in ELISA by neutralizing antibodies. It was transported to the plasma membrane of insect cells where its ability to induce membrane fusion was preserved. The efficacy of the recombinant protein as a vaccine was compared with those of an inactivated and an attenuated vaccine. When injected intraperitoneally into rainbow trout, the baculo-virus-encoded protein was shown (i) to induce the synthesis of VHSV-neutralizing antibodies and (ii) to confer protection against virus challenge. Immunization performed by immersion failed. This is the first report of a recombinant vaccine that protects fish against VHSV.
http://hdl.handle.net/2268/19856
10.1099/0022-1317-75-7-1579
http://vir.sgmjournals.org/cgi/content/abstract/75/7/1579

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