Reference : Functional analysis of ZNF85 KRAB zinc finger protein, a member of the highly homolog...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/19828
Functional analysis of ZNF85 KRAB zinc finger protein, a member of the highly homologous ZNF91 family
English
Poncelet, Dominique A. [> >]
Bellefroid, Eric J. [> > > >]
Bastiaens, P. V. [> > > >]
Demoitie, M. A. [> > > >]
Marine, J. C. [> > > >]
Pendeville-Samain, Hélène [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Alami, Y. [> > > >]
Devos, Nathalie mailto [> >]
Lecocq, P. [> >]
Ogawa, T. [> > > >]
Muller, Marc mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
1998
DNA & Cell Biology
Mary Ann Liebert, Inc.
17
11
931-43
Yes (verified by ORBi)
International
1044-5498
1557-7430
Larchmont
NY
[en] Amino Acid Sequence ; Animals ; Base Sequence ; COS Cells ; Cell Nucleus/metabolism ; DNA/metabolism ; DNA, Complementary ; DNA-Binding Proteins/biosynthesis/*chemistry/genetics/*physiology ; Gene Expression ; Humans ; Kruppel-Like Transcription Factors ; Male ; Molecular Sequence Data ; Protein Binding ; Recombinant Fusion Proteins/genetics ; Repressor Proteins/biosynthesis/genetics/*physiology ; Testis/*metabolism ; Zinc/metabolism ; Zinc Fingers/genetics/*physiology
[en] We previously identified the ZNF85 (HPF4) KRAB zinc finger gene, a member of the human ZNF91 family. Here, we show that the ZNF85 gene is highly expressed in normal adult testis, in seminomas, and in the NT2/D1 teratocarcinoma cell line. Immunocytochemical localization of a panel of beta-Gal/ZNF85 fusion proteins revealed that ZNF85 contains at least one nuclear localization signal located in the spacer region connecting the KRAB domain with the zinc finger repeats. Bacterially expressed ZNF85 zinc finger domain bound strongly and exclusively to DNA in vitro in a zinc-dependent manner. The KRAB(A) domain of the ZNF85 protein and of several other members of the ZNF91 family exhibited repressing activity when tested in Gal4 fusion protein assays. The repression was significantly enhanced by the addition of the KRAB (B) domain, whereas further addition of other conserved regions had no effect. The ZNF85 KRAB(A) and (B) domains in vitro bound several nuclear proteins that might constitute critical cofactors for repression.
http://hdl.handle.net/2268/19828
1998/12/05

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