No full text
Article (Scientific journals)
Functional analysis of ZNF85 KRAB zinc finger protein, a member of the highly homologous ZNF91 family
Poncelet, Dominique A.; Bellefroid, Eric J.; Bastiaens, P. V. et al.
1998In DNA and Cell Biology, 17 (11), p. 931-43
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Amino Acid Sequence; Animals; Base Sequence; COS Cells; Cell Nucleus/metabolism; DNA/metabolism; DNA, Complementary; DNA-Binding Proteins/biosynthesis/*chemistry/genetics/*physiology; Gene Expression; Humans; Kruppel-Like Transcription Factors; Male; Molecular Sequence Data; Protein Binding; Recombinant Fusion Proteins/genetics; Repressor Proteins/biosynthesis/genetics/*physiology; Testis/*metabolism; Zinc/metabolism; Zinc Fingers/genetics/*physiology
Abstract :
[en] We previously identified the ZNF85 (HPF4) KRAB zinc finger gene, a member of the human ZNF91 family. Here, we show that the ZNF85 gene is highly expressed in normal adult testis, in seminomas, and in the NT2/D1 teratocarcinoma cell line. Immunocytochemical localization of a panel of beta-Gal/ZNF85 fusion proteins revealed that ZNF85 contains at least one nuclear localization signal located in the spacer region connecting the KRAB domain with the zinc finger repeats. Bacterially expressed ZNF85 zinc finger domain bound strongly and exclusively to DNA in vitro in a zinc-dependent manner. The KRAB(A) domain of the ZNF85 protein and of several other members of the ZNF91 family exhibited repressing activity when tested in Gal4 fusion protein assays. The repression was significantly enhanced by the addition of the KRAB (B) domain, whereas further addition of other conserved regions had no effect. The ZNF85 KRAB(A) and (B) domains in vitro bound several nuclear proteins that might constitute critical cofactors for repression.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Poncelet, Dominique A.
Bellefroid, Eric J.
Bastiaens, P. V.
Demoitie, M. A.
Marine, J. C.
Pendeville-Samain, Hélène ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Alami, Y.
Devos, Nathalie
Lecocq, P.
Ogawa, T.
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Functional analysis of ZNF85 KRAB zinc finger protein, a member of the highly homologous ZNF91 family
Publication date :
1998
Journal title :
DNA and Cell Biology
ISSN :
1044-5498
eISSN :
1557-7430
Publisher :
Mary Ann Liebert, Inc., Larchmont, United States - New York
Volume :
17
Issue :
11
Pages :
931-43
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 August 2009

Statistics


Number of views
105 (17 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
23
Scopus citations®
without self-citations
20
OpenCitations
 
17

Bibliography


Similar publications



Contact ORBi