Title : Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp Language : English Author, co-author : Kausel, Gudrun [Universidad Austral de Chile > > > >] Vera, María Inés [Universidad Nacional Andrés Bello > > > >] San Martin, Rody [Universidad Austral de Chile > > > >] Figueroa, Jaime [Universidad Austral de Chile > > > >] Molina, Alfredo [> >] Muller, Marc [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >] Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >] Krauskopf, Manuel [Universidad Nacional Andrés Bello > > > >] Publication date : 9-Nov-1999 Journal title : Journal of Cellular Biochemistry Publisher : Wiley Liss, Inc. Volume : 75 Issue/season : 4 Pages : 598 - 609 Audience : International ISSN : 0730-2312 e-ISSN : 1097-4644 Keywords : [en] factor Pit-1 ; eurythermal ectotherms ; carpAbstract : [en] A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer-acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer-acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat. J. Cell. Biochem. 75:598-609, 1999. © 1999 Wiley-Liss, Inc.Permalink : http://hdl.handle.net/2268/19822 Other URL : http://www3.interscience.wiley.com/journal/66500659/abstract?CRETRY=1&SRETRY=0 
[Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
