Reference : Framework for the Integration of Genomics, Epigenomics and Transcriptomics in Complex...
Scientific journals : Article
Life sciences : Multidisciplinary, general & others
http://hdl.handle.net/2268/198219
Framework for the Integration of Genomics, Epigenomics and Transcriptomics in Complex Diseases.
English
Pineda, Silvia [> >]
Gomez-Rubio, Paulina [> >]
Picornell, Antonio [> >]
Bessonov, Kyrylo mailto [Université de Liège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Bioinformatique >]
Marquez, Mirari [> >]
Kogevinas, Manolis [> >]
Real, Francisco X. [> >]
Van Steen, Kristel mailto [Université de Liège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Bioinformatique >]
Malats, Nuria [> >]
2015
Human Heredity
79
3-4
124-36
Yes (verified by ORBi)
International
0001-5652
1423-0062
Switzerland
[en] Adult ; Aged ; Aged, 80 and over ; CpG Islands/genetics ; DNA Methylation/genetics ; Disease/genetics ; Epigenomics ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Gene Frequency/genetics ; Genome-Wide Association Study ; Genotyping Techniques ; Humans ; Male ; Middle Aged ; Polymorphism, Single Nucleotide/genetics ; Quantitative Trait Loci/genetics ; Statistics as Topic
[en] OBJECTIVES: Different types of '-omics' data are becoming available in the post-genome era; still a single -omics assessment provides limited insights to understand the biological mechanism of complex diseases. Genomics, epigenomics and transcriptomics data provide insight into the molecular dysregulation of neoplastic diseases, among them urothelial bladder cancer (UBC). Here, we propose a detailed analytical framework necessary to achieve an adequate integration of the three sets of -omics data to ultimately identify previously hidden genetic mechanisms in UBC. METHODS: We built a multi-staged framework to study possible pair-wise combinations and integrated the data in three-way relationships. SNP genotypes, CpG methylation levels and gene expression levels were determined for a total of 70 individuals with UBC and with fresh tumour tissue available. RESULTS: We suggest two main hypothesis-based scenarios for gene regulation based on the -omics integration analysis, where DNA methylation affects gene expression and genetic variants co-regulate gene expression and DNA methylation. We identified several three-way trans-association 'hotspots' that are found at the molecular level and that deserve further studies. CONCLUSIONS: The proposed integrative framework allowed us to identify relationships at the whole-genome level providing some new biological insights and highlighting the importance of integrating -omics data.
http://hdl.handle.net/2268/198219
10.1159/000381184
2015 S. Karger AG, Basel.

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