ORBi: Van de Weerdt Cécile - Far upstream sequences regulate the human prolactin promoter transcription

Reference : Far upstream sequences regulate the human prolactin promoter transcription


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/19821

Title :	Far upstream sequences regulate the human prolactin promoter transcription
Language :	English
Author, co-author :	Van de Weerdt, Cécile [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Peers, Bernard [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Belayew, A. [> > > >]
	Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Muller, Marc [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Publication date :	2000
Journal title :	Neuroendocrinology
Publisher :	Karger
Volume :	71
Issue/season :	2
Pages :	124-37
Audience :	International
ISSN :	0028-3835
e-ISSN :	1423-0194
City :	Basel
Country :	Switzerland
Keywords :	[en] Amino Acid Sequence ; Animals ; CCAAT-Enhancer-Binding Proteins ; DNA Footprinting ; DNA Primers ; DNA-Binding Proteins/analysis/genetics ; Gene Expression Regulation/genetics ; Hela Cells ; Humans ; Jurkat Cells ; Liver/chemistry ; Lymphocytes/physiology ; Molecular Sequence Data ; Nuclear Proteins/analysis/genetics ; Pituitary Gland/cytology/physiology ; Plasmids ; Prolactin/genetics ; Promoter Regions, Genetic/genetics ; Rats ; Transcriptional Activation/genetics
Abstract :	[en] The human prolactin gene is mainly expressed in pituitary lactotrope cells, but transcription from an alternative, far upstream promoter was detected in lymphoid, placental and mammary cells. We describe the transcriptional activity in rat pituitary cells of the complete region separating the two promoters, using transient transfection experiments. A far upstream activating region was only functional in combination with the prolactin promoter. DNaseI protection experiments revealed, in addition to binding sites for the pituitary-specific factor Pit-1, sites (e.g. SD1) for several ubiquitous factors and one lymphoid-specific factor (SD4). A single copy of the ubiquitous site SD1 or the lymphoid-specific site SD4 was unable to activate transcription of a heterologous promoter in pituitary cells. However, SD1 activated transcription in nonpituitary cells and SD4 was functional specifically in lymphoid cells. Five copies of a distal site (D8) activated transcription in each cell type tested. Gel retardation experiments show that this site binds the specific factor C/EBP in liver and a distinct factor in other cell types. Our results suggest that different elements within this large region direct specific expression from each promoter via a complex interplay between cell-specific and ubiquitous transcription factors.
Permalink :	http://hdl.handle.net/2268/19821
DOI :	10.1159/000054528
Other URL :	http://content.karger.com/produktedb/produkte.asp?typ=fulltext&file=nen71124
Mentions required by the publisher for OA :	2000/02/25

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