Article (Scientific journals)
Loss of nucleotide regulation of epithelial chloride transport in the jejunum of P2Y4-null mice
Robaye, B.; Ghanem, E.; Wilkin, F. et al.
2003In Molecular Pharmacology, 63 (4), p. 777-783
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Abstract :
[en] The P2Y4 receptor is responsive to UTP in human and to ATP and UTP in rodents. With the aim of identifying its pharmacotherapeutic interest, we generated P2Y4-null mice by a classic gene targeting method. The proportion of genotypes was consistent with X-linked Mendelian transmission. Gene inactivation was checked by the complete disappearance of P2Y4 receptor mRNA from liver, stomach, and intestine. The P2Y4-null mice had a grossly normal behavior, growth, and reproduction. Chloride secretion by the jejunal epithelium was assessed in Ussing chambers by the measurement of the short circuit current in the presence of phlorizin. We show here that the UTP- and ATPinduced chloride secretory responses observed in wild-type mice are abolished in P2Y4-null mice. This is the first clearcut demonstration of a biological role of the P2Y4 receptor
Disciplines :
Biochemistry, biophysics & molecular biology
Genetics & genetic processes
Author, co-author :
Robaye, B.;  Université Libre de Bruxelles - ULB > Institute of Interdisciplinary Research, Institute of Biology and Molecular Medicine
Ghanem, E.;  Université Libre de Bruxelles - ULB > Laboratory of Physiopathology, School of Medicine
Wilkin, F.;  Université Libre de Bruxelles - ULB > Institute of Interdisciplinary Research, Institute of Biology and Molecular Medicine
Fokan, D.;  Université Libre de Bruxelles - ULB > Institute of Interdisciplinary Research, Institute of Biology and Molecular Medicine
Van Driessche, W.;  Université Catholique de Louvain - UCL > Department of Physiology
Schurmans, Stéphane  ;  Université Libre de Bruxelles - ULB > Institut de Recherche Interdisciplinaire en Biologie Humaine et Moleculaire
Boeynaems, J. M.;  Université Libre de Bruxelles - ULB > Institute of Interdisciplinary Research, Institute of Biology and Molecular Medicine
Beauwens, R.;  Université Libre de Bruxelles - ULB > Laboratory of Physiopathology, School of Medicine
Language :
English
Title :
Loss of nucleotide regulation of epithelial chloride transport in the jejunum of P2Y4-null mice
Publication date :
2003
Journal title :
Molecular Pharmacology
ISSN :
0026-895X
eISSN :
1521-0111
Publisher :
American Society for Pharmacology and Experimental Therapeutics, Bethesda, United States - Maryland
Volume :
63
Issue :
4
Pages :
777-783
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Fonds Forton, an Action de Recherche Concertée of the Communauté Française de Belgique, the Belgian Program on Interuniversity Poles of Attraction initiated by the Belgian State, Prime Minister’s Office, Federal Service for Science, Technology and Culture, grants of the Fonds de la Recherche Scientifique Médicale, and the Fonds Emile DEFAY
Commentary :
ABBREVIATIONS: bp, base pair(s); PCR, polymerase chain reaction; RT, reverse transcription; HPRT, hypoxanthine phosphoribosyltransferase; SSC, short-circuit current; 8-p-SPT, 8-(para-sulfophenyl)theophylline; ES, embryonic stem; CFTR, cystic fibrosis transmembrane conductance regulator.
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