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Interactions between Lipopolysaccharides and Blood Factors on the Stimulation of Equine Polymorphonuclear Neutrophils
Benbarek, H.; Deby-Dupont, G.; Caudron, I. et al.
1998In Veterinary Immunology and Immunopathology, 64 (4), p. 313-22
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Abstract :
[en] In horses, the mechanisms of lipopolysaccharide (LPS) stimulation of isolated neutrophils to produce reactive oxygen species remain unknown. We re-investigated this problem by monitoring the luminol-enhanced chemiluminescence (CL) produced by LPS-stimulated equine neutrophils. The neutrophils were isolated from horse blood by discontinuous density gradient centrifugation (> or = 99% neutrophils; viability > or = 98%). Increasing concentrations of Escherichia coli (E. coli) LPS (from 0.01-10 microg ml(-1)) were used to activate the neutrophils. When LPS was used directly, without another stimulator, the respiratory burst of neutrophils was not activated (N=12 horses; n=5 assays per horse). On the contrary, when LPS was added to whole blood, the neutrophils isolated from this blood were stimulated in a LPS dose-dependent manner, but polymyxin B added to whole blood suppressed this stimulation (N=2; n=6). LPS dissolved in autologous equine plasma stimulated the isolated neutrophils in a dose-dependent manner from 0.1-10 microg ml(-1) (N=5; n=12). Heat inactivation of the plasma abolished this CL increase (N=2; n=5). LPS added to equine albumin did not stimulate the isolated neutrophils (N=2; n=5). On the contrary, the addition of gamma-globulins (1 mg ml(-1)) to LPS (10 microg ml(-1)) led to the stimulation of neutrophils (N=2; n=5). We concluded that LPS did not directly stimulate the isolated equine neutrophils, but that plasmatic factors are needed for the stimulation of these cells by LPS.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Benbarek, H.
Deby-Dupont, G.
Caudron, I.
Grulke, Sigrid  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Deby, Christiane ;  Centre Hospitalier Universitaire de Liège - CHU > Administration des patients - Admission des hospitalisés
Lamy, Maurice ;  Université de Liège - ULiège > Département des sciences cliniques > Anesthésie et réanimation
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Interactions between Lipopolysaccharides and Blood Factors on the Stimulation of Equine Polymorphonuclear Neutrophils
Publication date :
31 August 1998
Journal title :
Veterinary Immunology and Immunopathology
ISSN :
0165-2427
eISSN :
1873-2534
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
64
Issue :
4
Pages :
313-22
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 May 2016

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