Poster (Scientific congresses and symposiums)
Peptidoglycan fragments separation by CE/LC-MS
Boulanger, Madeleine; Delvaux, Cédric; Far, Johann et al.
2016Seventh general assembly of the IAP P7/44 project "Integrative Protein Science (iPROS)"
 

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Keywords :
Beta-lactamase induction; Peptidoglycan; Liquid Chromatography; Capillary Electrophoresis; Mass spectrometry; ZIC-HILIC
Abstract :
[en] In Bacillus licheniformis 749/I, the induction of BlaP beta-lactamase relies on a complex regulation system. During this process, the intracellular repressor BlaI is inactivated by a dipeptide coming from the peptidoglycan (PG) degradation via an “AND Gate” regulation. This regulation involves the cellular stress induced by the beta-lactam, the membrane receptor BlaR1 and the PG turnover. Briefly, the induction occurs when the extracellular domain of BlaR1 is acylated by the antibiotic which leads to a reorganization of the transmembrane segments and the receptor autocleavage. Simultaneously, the beta-lactam partially inhibits the penicillin-binding protein 1 (PBP1), triggering increased PG turnover and accumulation of PG fragments. Some of these fragments could enter in the cytoplasm and undergo enzymatic degradations which lead to the formation of the pro co-activator (tripeptide L-Ala-D-Glu-m-A2pm). This pro co-activator generates the co-activator, the dipeptide D-Glu-m-A2pm. Nowadays the nature and the concentration of PG fragments inside the cytoplasm are unknown. Therefore, the development of different analytical methods is required in order to identify those cytoplasmic fragments. In this poster, three different ways to separate PG fragments are discussed.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Chemistry
Biochemistry, biophysics & molecular biology
Author, co-author :
Boulanger, Madeleine ;  Université de Liège > Département des sciences de la vie > Physiologie et génétique bactériennes
Delvaux, Cédric  ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Far, Johann  ;  Université de Liège > Département de chimie (sciences) > Center for Analytical Research and Technology (CART)
De Pauw, Edwin  ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Joris, Bernard ;  Université de Liège > Département des sciences de la vie > Physiologie et génétique bactériennes
Language :
English
Title :
Peptidoglycan fragments separation by CE/LC-MS
Publication date :
24 May 2016
Number of pages :
A0
Event name :
Seventh general assembly of the IAP P7/44 project "Integrative Protein Science (iPROS)"
Event organizer :
Université Catholique de Louvain
Patrice Soumillon
Bernard Joris
Event place :
Louvain-la-Neuve, Belgium
Event date :
24 mai 2016
Funders :
ULiège - Université de Liège [BE]
Available on ORBi :
since 20 May 2016

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