Article (Scientific journals)
Unraveling the complexity of the interactions of DNA nucleotides with gold by single molecule force spectroscopy
Bano, Fouzia; Sluysmans, Damien; Wislez, Arnaud et al.
2015In Nanoscale, 7, p. 19528–19533
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Keywords :
AFM; DNA
Abstract :
[en] Addressing the effect of different environmental factors on the adsorption of DNA to solid supports is critical for the development of robust miniaturized devices for applications ranging from biosensors to next generation molecular technology. Most of the time, thiol-based chemistry is used to anchor DNA on gold – a substrate commonly used in nanotechnology – and little is known about the direct interaction between DNA and gold. So far there have been no systematic studies on the direct adsorption behavior of the deoxyribonucleotides (i.e., a nitrogenous base, a deoxyribose sugar, and a phosphate group) and on the factors that govern the DNA–gold bond strength. Here, using single molecule force spectroscopy, we investigated the interaction of the four individual nucleotides, adenine, guanine, cytosine, and thymine, with gold. Experiments were performed in three salinity conditions and two surface dwell times to reveal the factors that influence nucleotide–Au bond strength. Force data show that, at physiological ionic strength, adenine–Au interactions are stronger, asymmetrical and independent of surface dwell time as compared to cytosine–Au and guanine–Au interactions. We suggest that in these conditions only adenine is able to chemisorb on gold. A decrease of the ionic strength significantly increases the bond strength for all nucleotides. We show that moderate ionic strength along with longer surface dwell period suggest weak chemisorption also for cytosine and guanine.
Disciplines :
Chemistry
Author, co-author :
Bano, Fouzia;  Université de Liège
Sluysmans, Damien  ;  Université de Liège > Département de chimie (sciences) > Nanochimie et systèmes moléculaires
Wislez, Arnaud ;  Université de Liège > Département de chimie (sciences) > Nanochimie et systèmes moléculaires
Duwez, Anne-Sophie  ;  Université de Liège > Département de chimie (sciences) > Nanochimie et systèmes moléculaires
Language :
English
Title :
Unraveling the complexity of the interactions of DNA nucleotides with gold by single molecule force spectroscopy
Publication date :
2015
Journal title :
Nanoscale
ISSN :
2040-3364
eISSN :
2040-3372
Publisher :
RSC Publishing, Cambridge, United Kingdom
Volume :
7
Pages :
19528–19533
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 May 2016

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