enzymatic extracts; chitinase; lysozyme activities; colloidal chitin; purification process; purification yield; electrophoretic analysis of "purified" fractions; enzymatic specificity of the purified preparations
Abstract :
[en] 1. Enzymatic extracts of Perodicticus potto gastric mucosa showed chitinolytic activity together with lysozymic activity. 2. After purification by adsortion on colloidal chitin and gel chromatography, an enzymatic preparation with high chitinolytic activity was devoid of any lysozymic activity. 3. The gastric chitinolytic enzymes of Perodicticus potto are thus "true" chitinases specific of the B-1,4-N-acetylglucosamine linkages in chitin molecule.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Cornelius, C.; Université de Liège - ULiège > Zoological institute - Institute L. Fredericq > Laboratories of morphology, systematics and animal ecology
Dandrifosse, Guy ; Université de Liège > Zoological institute - Institute L. Fredericq > Laboratories of morphology, systematics and animal ecology
Jeuniaux, Charles ; Université de Liège - ULiège > Zoological institute - Institute L. Fredericq > Laboratories of morphology, systematics and animal ecology
Language :
English
Title :
Chitinolytic enzymes of the gastric mucosa of Perodicticus Potto (Primate Prosimian) : purification and enzyme specificity
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