Poster (Scientific congresses and symposiums)
Hemipteran - host plant interactions: focus on some model insect saliva
Serteyn, Laurent; Moreau, Sophie; Delatour, Anaïs et al.
2015UK – French joint meeting on Aphids
 

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Keywords :
Hemiptera; Interactions; Saliva
Abstract :
[en] Insect salivary components play important roles in plant-insect interactions, stimulating the plant defence or promoting infestation by manipulating plant physiology. Indeed, a variety of enzymes and organic components in saliva of herbivory insects can induce series of biochemical responses in damaged plants. In our lab, several studies have been screening proteins in different aphid species saliva. The purpose of our current and future works is to widen that field of study to other piercing-sucking pests and host plants models. Three models will be compared: the pea aphid (Acyrtosiphon pisum Harris), the invasive Asian brown marmorated stink bug (Halyomorpha halys Stål) and the invasive South American green vegetable stink bug (Nezara viridula L.). A preliminary study showed differential expressions in bacterial proteins in salivary glands when N. viridula were exposed to tomato with or without glandular trichomes. This led to questioning whether the internal symbionts influence the adaptability of the pest to the host by manipulating saliva compounds. Then, a study can be led to assess the impact of symbiont profiles on pea aphid saliva proteins. We also began to analyse the impact of several diets on stink bugs salivary glands proteome. We use especially proteomic techniques, such as SDS-PAGE and 2D-DIGE followed by LC-MS/MS and MALDI-TOF analysis, but also liquid chromatography gel free approaches. The perspective would be applying saliva or salivary glands extracts on plant in order to screen its defensive responses by complementary “omic” approaches and, in term, to identify candidate proteins playing a role in plant defence induction or bypass.
Disciplines :
Entomology & pest control
Author, co-author :
Serteyn, Laurent ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Entomologie fonctionnelle et évolutive
Moreau, Sophie
Delatour, Anaïs
Francis, Frédéric  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Entomologie fonctionnelle et évolutive
Language :
English
Title :
Hemipteran - host plant interactions: focus on some model insect saliva
Publication date :
November 2015
Event name :
UK – French joint meeting on Aphids
Event organizer :
Biologie Adaptative des Pucerons et Organismes Associés (BAPOA)
Event place :
Paris, France
Event date :
5-6 novembre 2015
Audience :
International
Available on ORBi :
since 08 May 2016

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