ORBi: Noël Agnès - Antagonistic Effects of Laminin and Fibronectin in Cell-to-Cell and Cell-to-Matrix Interactions in Mcf-7 Cultures

Reference : Antagonistic Effects of Laminin and Fibronectin in Cell-to-Cell and Cell-to-Matrix Inter...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/19554

Title :	Antagonistic Effects of Laminin and Fibronectin in Cell-to-Cell and Cell-to-Matrix Interactions in Mcf-7 Cultures
Language :	English
Author, co-author :	Noël, Agnès [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme >]
	Calle, A. [> > > >]
	Emonard, H. [> > > >]
	Nusgens, Betty [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Protéines et glycoprot. de matr.extracell. et membran.basal. >]
	Foidart, Jean-Michel [Université de Liège - ULg > Département des sciences cliniques > Gynécologie - Obstétrique >]
	Lapiere, C. M. [> > > >]
Publication date :	May-1988
Journal title :	In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
Publisher :	Tissue Culture Association
Volume :	24
Issue/season :	5
Pages :	373-80
Audience :	International
ISSN :	0883-8364
City :	Gaithersburg
Country :	MD
Abstract :	[en] During morphogenesis, tumor progression and metastasis, cell adhesion, dissociation, and migration result from a complex balance between cell-to-cell and cell-to-matrix interactions. Two different organization patterns of MCF-7 cells were induced by different extracellular matrix proteins. When plated on plastic or polymeric type I collagen gel used as a model of interstitial matrix, MCF-7 cells spread and grew in monolayer. When cultured on a solid gel of basement membrane (BM) proteins (85% laminin) used as a model of BM, cells formed clusters attached to the matrix. Matrix proteins regulated these two types of cell organization by preferentially promoting cell-to-cell or cell-support interactions. On plastic in the presence of soluble laminin or on laminin-coated dishes, cells also formed clusters. Addition of soluble fibronectin induced spreading of the cells, suggesting that laminin and fibronectin have competitive antagonistic effects on MCF-7 cell morphology. Antilaminin antibodies inhibited cluster formation and attachment, emphasizing the important role of this glycoprotein not only in promoting cluster attachment but also in cell-to-cell contact formation. Such effects of extracellular matrix proteins could play significant roles in tumor progression and metastasis.
Permalink :	http://hdl.handle.net/2268/19554
	also: http://hdl.handle.net/2268/40005

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