Delayed neuroendocrine sexual maturation in female rats after a very low dose of Bisphenol A through altered GABAergic neurotransmission and opposing effects of a high dose.

Franssen, Delphine; GERARD, Arlette; HENNUY, Benoit; Donneau, Anne-Françoise; Bourguignon, Jean-Pierre; Parent, Anne-Simone

doi:10.1210/en.2015-1937

Article (Scientific journals)

Delayed neuroendocrine sexual maturation in female rats after a very low dose of Bisphenol A through altered GABAergic neurotransmission and opposing effects of a high dose.

Franssen, Delphine; GERARD, Arlette; HENNUY, Benoit et al.

2016 • In Endocrinology

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/195530

DOI
10.1210/en.2015-1937

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26950200

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Abstract :

[en] Rat sexual maturation is preceded by a reduction of the interpulse interval (IPI) of gonadotropinreleasing hormone (GnRH) neurosecretion. This work aims at studying disruption of that neuroendocrine event in females after early exposure to a very low dose of Bisphenol A (BPA), a ubiquitous endocrine disrupting chemical. Female rats were exposed to vehicle or BPA 25 ng/kg.day, 25 g/kg.day, or 5 mg/kg.day from postnatal day (PND) 1 to 5 or 15. Exposure to 25 ng/kg.day of BPA for 5 or 15 days was followed by a delay in developmental reduction of GnRH IPI studied ex vivo on PND 20. After 15 days of exposure to that low dose of BPA, vaginal opening tended to be delayed. In contrast, exposure to BPA 5 mg/kg.day for 15 days resulted in a premature reduction inGnRHIPI and a trend toward early vaginal opening. RNAseq analysis on PND20 indicated that exposure to BPA resulted in opposing dose effectsonthemRNAexpression of hypothalamic genes involved inGABAA neurotransmission. The study of GnRH secretion in vitro in the presence of GABAA receptor agonist/antagonist confirmed an increased or a reduced GABAergic tone after in vivo exposure to the very low or the high dose of BPA, respectively. Overall, we show for the first time that neonatal exposure to BPA leads to opposing dose-dependent effects on the neuroendocrine control of puberty in the female rat. A very low and environmentally relevant dose of BPA delays neuroendocrine maturation related to puberty through increased inhibitory GABAergic neurotransmission.

Disciplines :

Endocrinology, metabolism & nutrition

Author, co-author :

Franssen, Delphine ; Université de Liège > Département des sciences cliniques > Pédiatrie

GERARD, Arlette ; Centre Hospitalier Universitaire de Liège - CHU > Service de pédiatrie

HENNUY, Benoit ; Centre Hospitalier Universitaire de Liège - CHU > Centre d'oncologie

Donneau, Anne-Françoise ; Université de Liège > Département des sciences de la santé publique > Biostatistique

Bourguignon, Jean-Pierre ; Université de Liège > Département des sciences cliniques > Département des sciences cliniques

Parent, Anne-Simone ; Université de Liège > Département des sciences cliniques > Pédiatrie

Language :

English

Title :

Delayed neuroendocrine sexual maturation in female rats after a very low dose of Bisphenol A through altered GABAergic neurotransmission and opposing effects of a high dose.

Publication date :

2016

Journal title :

Endocrinology

ISSN :

0013-7227

eISSN :

1945-7170

Publisher :

Endocrine Society, Chevy Chase, United States - Maryland

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 12 April 2016

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