Article (Scientific journals)
A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials.
GRENADE, Charlotte; Moniotte, Nicolas; Rompen, Eric et al.
2016In Cytotechnology
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Keywords :
Biomaterials; Cytocompatibility; Dental prostheses; Human gingival fibroblasts; Insert; Membranes
Abstract :
[en] In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to study cell behavior on rigid and flexible/permeable biomaterials elaborating two specific insert-based systems (IBS-R and IBS-F respectively). IBS-R was designed as a specific cylindrical polytetrafluoroethylene (PTFE) system to evaluate attachment, proliferation and morphology of human gingival fibroblasts (HGFs) on grade V titanium and lithium disilicate glass-ceramic discs characteristics of dental prostheses. The number of cells, their covering on discs and their morphology were determined from MTS assays and microscopic fluorescent images after 24, 48 and 72 h. IBS-F was developed as a two components system to study HGFs behavior on guided bone regeneration polyester membranes. The viability and the membrane barrier effect were evaluated by metabolic MTS assays and by scanning electron microscopy. IBS-R and IBS-F were shown to promote (1) easy and rapid handling; (2) cell retention on biomaterial surface; (3) accurate evaluation of the cellular proliferation, spreading and viability; (4) use of non-toxic material. Moreover IBS-F allowed the study of the cell migration through degradable membranes, with an access to both faces of the biomaterial and to the bottom of culture wells for medium changing.
Research center :
d‐BRU - Dental Biomaterials Research Unit - ULiège [BE]
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Dentistry & oral medicine
Biotechnology
Materials science & engineering
Author, co-author :
GRENADE, Charlotte ;  Centre Hospitalier Universitaire de Liège - CHU > Service prothèse fixée
Moniotte, Nicolas
Rompen, Eric ;  Université de Liège > Département de sciences dentaires > Chirurgie bucco-dentaire et parodontologie
Vanheusden, Alain ;  Université de Liège > Département de sciences dentaires > Prothèse dentaire fixe - Anatomie bucco-dentaire
Mainjot, Amélie  ;  Université de Liège > Département de sciences dentaires > Biomatériaux dentaires
De Pauw-Gillet, Marie-Claire ;  Université de Liège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Language :
English
Title :
A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials.
Publication date :
25 March 2016
Journal title :
Cytotechnology
ISSN :
0920-9069
eISSN :
1573-0778
Publisher :
Kluwer Academic Publishers, Netherlands
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 April 2016

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