Article (Scientific journals)
Structural Proteomics of Herpesviruses.
Leroy, Baptiste; Gillet, Laurent; Vanderplasschen, Alain et al.
2016In Viruses, 8 (2)
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Keywords :
herpesvirus; host proteins; proteomic; structural proteins
Abstract :
[en] Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have been unsuccessful because these viruses have developed numerous immune evasion mechanisms. Therefore, a better understanding of their complex lifecycle is needed. In particular, while the genome of numerous herpesviruses has been sequenced, the exact composition of virions remains unknown for most of them. Mass spectrometry has recently emerged as a central method and has permitted fundamental discoveries in virology. Here, we review mass spectrometry-based approaches that have recently allowed a better understanding of the composition of the herpesvirus virion. In particular, we describe strategies commonly used for proper sample preparation and fractionation to allow protein localization inside the particle but also to avoid contamination by nonstructural proteins. A collection of other important data regarding post-translational modifications or the relative abundance of structural proteins is also described. This review also discusses the poorly studied importance of host proteins in herpesvirus structural proteins and the necessity to develop a quantitative workflow to better understand the dynamics of the structural proteome. In the future, we hope that this collaborative effort will assist in the development of new strategies to fight these infections.
Disciplines :
Microbiology
Author, co-author :
Leroy, Baptiste
Gillet, Laurent  ;  Université de Liège - ULiège
Vanderplasschen, Alain ;  Université de Liège > Immunologie et vaccinologie
Wattiez, Ruddy
Language :
English
Title :
Structural Proteomics of Herpesviruses.
Publication date :
2016
Journal title :
Viruses
eISSN :
1999-4915
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
Volume :
8
Issue :
2
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 March 2016

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