Article (Scientific journals)
Experimental Neospora caninum infection modifies trophoblast cell populations and plasma pregnancy-associated glycoprotein 1 and 2 dynamics in pregnant dairy heifers.
Mur-Novales, R.; Serrano-Perez, B.; Garcia-Ispierto, I. et al.
2016In Veterinary Parasitology, 216, p. 7-12
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Keywords :
Bovine neosporosis; Mono- and binucleate cells; PAGs; Placental damage
Abstract :
[en] Neospora caninum is an obligate intracellular protozoan that causes abortion in cattle worldwide. Plasma concentrations of pregnancy-associated glycoprotein 1 (PAG-1), produced by binucleate trophoblast cells, are used for pregnancy diagnosis and as a marker of foetal-placental well-being, while PAG-2, produced by both mono- and binucleate trophoblast cells, may serve as an indicator of abortion risk. In prior work, natural N. caninum infection was found to modify plasma PAG-1 and PAG-2 patterns. The present study examines PAG-1 and PAG-2 dynamics and trophoblast cell populations following experimental infection with N. caninum. The study population was comprised of 17 N. caninum seronegative Holstein-Friesian heifers. On day 110 of gestation, 6 heifers were inoculated intravenously with 10(7) taquyzoites of N. canimum. Plasma samples for PAG-1 and PAG-2 determinations were collected on days 0, 7, 14, 21 and 42 post infection. During the study course, pregnancy was normally expressed in all controls while three infected heifers lost their foetuses. All heifers were euthanised on day 42 post infection and placentome samples from the 14 non-aborting heifers were collected to examine trophoblast cell populations. Plasma PAG-1 and PAG-2 concentrations in non-infected heifers increased until the day of euthanasia while non-aborting infected heifers showed a temporary fall in PAG-1 (P<0.004) and PAG-2 (P<0.002) concentrations from 7 to 14 days post infection (dpi). The two dams aborting at 14 and 21 dpi and a third dam with a mummified foetus upon euthanasia showed dramatic PAG-1 and PAG-2 reductions from 14 dpi to undetectable levels upon euthanasia. A stereological study of placentomes revealed significantly higher relative proportions of mono- (P=0.035) and binucleate (P=0.029) trophoblast cells at 42 dpi in non-infected heifers than infected non-aborting heifers. According to our findings, following experimental N. caninum infection on day 110 of gestation, non-aborting heifers showed a brief reversible drop in plasma PAG-1 and 2 concentrations two weeks later and reduced proportions of bi- and mono-nucleate trophoblast cells 42 days after infection. In aborting dams, dramatically reduced PAG levels were related to severe placental damage and a non-viable pregnancy.
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Mur-Novales, R.;  University of Lleida, Spain > Department of Animal Production
Serrano-Perez, B.;  University of Lleida, Spain > Department of Animal Production
Garcia-Ispierto, I.;  University of Lleida, Spain > Department of Animal Production
Melo de Sousa, Noelita ;  Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Beckers, Jean-François  ;  Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Almeria, S.;  Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Barcelona, Spain > Departament de Sanitat i Anatomia Animals
Lopez-Gatius, F.;  University of Lleida, Spain > Agrotecnio Centre
Language :
English
Title :
Experimental Neospora caninum infection modifies trophoblast cell populations and plasma pregnancy-associated glycoprotein 1 and 2 dynamics in pregnant dairy heifers.
Publication date :
2016
Journal title :
Veterinary Parasitology
ISSN :
0304-4017
eISSN :
1873-2550
Publisher :
Elsevier, Netherlands
Volume :
216
Pages :
7-12
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
This study was supported by a grant from the SpanishMINECO (AGL2012-39830-C02-01/02) and FEDER. Ramón Mur-Novales was supported by a FPI grant from the Spanish Ministryof Science and Innovation, MICINN, BES-2013-063215.
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since 03 March 2016

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