Article (Scientific journals)
Characterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD
Odjo, Djosse Psijus Sylvanus; Bera, François; Jacquet, Nicolas et al.
2016In Stärke, 68, p. 1-9
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Keywords :
Corn / Digestion / HPAEC-PAD; Oligosaccharides / Starch
Abstract :
[en] Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified and characterized using high-performance anion-exchange chromatography coupled with a pulsed amperometric detector (HPAEC-PAD) and two colorimetric approaches (glucose oxidase/ peroxidase assay and the 3,5-dinitrosalicylic acid assay). HPAEC-PAD revealed five major saccharides as the result of in vitro pepsin-pancreatin digestion of corn grain: glucose, isomaltose, maltose, maltotriose, and glucosyl-maltotriose. Concentrations of glucose released as measured by the three methods assessed are similar when the in vitro pepsin-pancreatin digestion is followed by post incubation with amyloglucosidase. This post incubation unfortunately leads to a loss of information about the degree of polymerization of oligosaccharides that can impact their absorption through intestinal tract. HPAEC-PAD gives both qualitative and quantitative information and then seems more suitable for a nutritional appreciation of the digestion of starchy feedstuffs.
Disciplines :
Food science
Author, co-author :
Odjo, Djosse Psijus Sylvanus ;  Université de Liège - ULiège > Doct. sc. agro. & ingé. biol.
Bera, François ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Labo. d'Ingénierie des procédés agro-alim.
Jacquet, Nicolas  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle
Richel, Aurore  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle
Malumba Kamba, Paul  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Labo. d'Ingénierie des procédés agro-alim.
Language :
English
Title :
Characterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD
Publication date :
2016
Journal title :
Stärke
ISSN :
0038-9056
eISSN :
1521-379X
Publisher :
Wiley-VCH
Volume :
68
Pages :
1-9
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
MAISCVOL
Funders :
Direction Génerale Opérationnelle de l’Agriculture, des Ressources Naturelles et de l’Environnement
Available on ORBi :
since 01 March 2016

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