[en] Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified
and characterized using high-performance anion-exchange chromatography coupled with a
pulsed amperometric detector (HPAEC-PAD) and two colorimetric approaches (glucose oxidase/
peroxidase assay and the 3,5-dinitrosalicylic acid assay). HPAEC-PAD revealed five major
saccharides as the result of in vitro pepsin-pancreatin digestion of corn grain: glucose,
isomaltose, maltose, maltotriose, and glucosyl-maltotriose. Concentrations of glucose released as
measured by the three methods assessed are similar when the in vitro pepsin-pancreatin
digestion is followed by post incubation with amyloglucosidase. This post incubation
unfortunately leads to a loss of information about the degree of polymerization of oligosaccharides that can impact their absorption through intestinal tract. HPAEC-PAD gives both qualitative and quantitative information and then seems more suitable for a nutritional appreciation of the digestion of starchy feedstuffs.
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