Article (Scientific journals)
Chromogenic detection of aminoglycoside phosphotransferases.
Amoroso, Ana Maria; Gutkind, G. O.
2001In Methods in Molecular Medicine, 48, p. 113-7
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Abstract :
[en] Acquired resistance to aminoglycosides is most frequently due to the presence of the so-called aminoglycoside modifying enzymes (AGME) (1) able to catalyze one or more of three general reactions: N-acetylation, O-nucleotidylation and O-phosphorylation (2). Although resistance phenotype (to different (substrate or not for enzymatic modification) may serve as an approach for identifying actual enzymes present in a given isolate (3), results can be obscured or confusing, particularly when several different enzymes (4) (even, isoenzymes with different affinities) are superimposing their action in a single microorganism with potential "permeability" or target alterations. Thus, identification of the AGME content of a given strain also requires screening at the DNA level using probes specific to all the known AGME (5). However, the complete set of probes is available only to a few laboratories around the world, making surveillance for the appearance of novel enzymes, or the unlikely evolution of those already known, a relatively nonfeasible goal, as search for new enzymes may begin only after failing to hybridize to all known probes.
Disciplines :
Microbiology
Author, co-author :
Amoroso, Ana Maria ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Gutkind, G. O.
Language :
English
Title :
Chromogenic detection of aminoglycoside phosphotransferases.
Publication date :
2001
Journal title :
Methods in Molecular Medicine
ISSN :
1543-1894
Publisher :
Humana Press, United States
Volume :
48
Pages :
113-7
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 29 February 2016

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