Article (Scientific journals)
Production and Characterization of Monoclonal Antibodies to Bovid Herpesvirus-4
Dubuisson, J.; Thiry, Etienne; Bublot, M. et al.
1989In Veterinary Microbiology, 19 (4), p. 305-15
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Abstract :
[en] Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpesvirus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma cells with splenocytes of BALB/c mice previously immunized with purified BHV-4. A modified indirect fluorescent antibody test (IFAT) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. The selected Mabs were tested by the same IFAT against a panel of BHV-4 field isolates and against bovine herpesvirus-1, bovine herpesvirus-2 and alcelaphine herpesvirus-1 (AHV-1). Comparison of BHV-4 field isolates with Mabs confirmed their close antigenic relationships, but slight antigenic differences were observed between different isolates. One of the Mabs also reacted against AHV-1, indicating an antigenic relationship between BHV-4 and AHV-1. None of the Mabs reacting with BHV-4 possessed neutralizing activity against the strain used for immunization.
Disciplines :
Microbiology
Veterinary medicine & animal health
Author, co-author :
Dubuisson, J.
Thiry, Etienne ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Bublot, M.
Sneyers, M.
Boulanger, D.
Guillaume, José
Pastoret, Paul-Pierre ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires
Language :
English
Title :
Production and Characterization of Monoclonal Antibodies to Bovid Herpesvirus-4
Publication date :
April 1989
Journal title :
Veterinary Microbiology
ISSN :
0378-1135
eISSN :
1873-2542
Publisher :
Elsevier, Netherlands
Volume :
19
Issue :
4
Pages :
305-15
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 August 2009

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