Reference : Production and Characterization of Monoclonal Antibodies to Bovid Herpesvirus-4
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/19399
Production and Characterization of Monoclonal Antibodies to Bovid Herpesvirus-4
English
Dubuisson, J. [> > > >]
Thiry, Etienne mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
Bublot, M. [> > > >]
Sneyers, M. [> > > >]
Boulanger, D. [> > > >]
Guillaume, José [ > > ]
Pastoret, Paul-Pierre mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires >]
Apr-1989
Veterinary Microbiology
19
4
305-15
Yes (verified by ORBi)
International
0378-1135
[en] Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpesvirus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma cells with splenocytes of BALB/c mice previously immunized with purified BHV-4. A modified indirect fluorescent antibody test (IFAT) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. The selected Mabs were tested by the same IFAT against a panel of BHV-4 field isolates and against bovine herpesvirus-1, bovine herpesvirus-2 and alcelaphine herpesvirus-1 (AHV-1). Comparison of BHV-4 field isolates with Mabs confirmed their close antigenic relationships, but slight antigenic differences were observed between different isolates. One of the Mabs also reacted against AHV-1, indicating an antigenic relationship between BHV-4 and AHV-1. None of the Mabs reacting with BHV-4 possessed neutralizing activity against the strain used for immunization.
http://hdl.handle.net/2268/19399

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