Article (Scientific journals)
Synthesis and Processing of Bovine Herpesvirus-1 Glycoprotein H
Baranowski, Eric; Dubuisson, Jean; van Drunen Little-van den Hurk, Sylvia et al.
1995In Virology, 206 (1), p. 651-4
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Abstract :
[en] The translation product of the bovine herpesvirus-1 (BHV-1) gH gene was identified and characterized. Synthetic peptides were used to generate specific antisera and a glycoprotein of 108K was precipitated by one of the antisera. Cross-immunoprecipitations with monoclonal antibodies to BHV-1 glycoprotein gp108 and the anti-gH peptide antiserum demonstrated that gp108 is the translation product of the gH open reading frame. Glycoprotein gH synthesis and intracellular processing was analyzed in infected Madin-Darby bovine kidney cells using anti-gp 108 monoclonal antibodies. Glycoprotein gH is expressed as a beta-gamma protein and could be detected by radioimmunoprecipitation as early as 2 hr postinfection. Cotranslational N-glycosylation of gH is essential for the recognition by monoclonal antibodies, suggesting that N-linked glycans are involved in protein folding or that they are targets for most of monoclonal antibodies used in this study.
Disciplines :
Microbiology
Veterinary medicine & animal health
Author, co-author :
Baranowski, Eric
Dubuisson, Jean
van Drunen Little-van den Hurk, Sylvia
Babiuk, A. Lorne
Michel, Alain
Pastoret, Paul-Pierre ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires
Thiry, Etienne ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Language :
English
Title :
Synthesis and Processing of Bovine Herpesvirus-1 Glycoprotein H
Publication date :
10 January 1995
Journal title :
Virology
ISSN :
0042-6822
eISSN :
1096-0341
Publisher :
Elsevier, Atlanta, United States - California
Volume :
206
Issue :
1
Pages :
651-4
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 August 2009

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