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Poster (Scientific congresses and symposiums)
Myoclonin 1 modulates the post-translational modification of microtubules
Medard, Laurie; Lakaye, Bernard; Godin, Juliette et al.
2015BSCDB SPRING MEETING 2015
 

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Keywords :
Myoclonin 1; Microtubules; post-translational modification
Abstract :
[en] Juvenile myoclonic epilepsy is one of the most common forms of generalized genetic epilepsy. Genetic studies have shown that heterozygous mutations in Myoclonin1 are responsible for 3-9% of clinical cases worldwide. This protein contains three DM10 domains of unknown function and an EF-hand domain. We have previously demonstrated that Myoclonin1 is a microtubule-associated protein involved in cell division and radial migration during neocortex development. In cells, this protein co-localized with specific structures rich in microtubules (MTs) such as the centrosome, the poles of the mitotic spindle or the motile cilia but not with cytoplasmic MTs. This suggests post-translational modifications (PTM) of MTs may be important for the interaction between Myoclonin1 and MTs. We have co-express the different enzymes catalyzing PTM of MTs with Myoclonin1 in U2OS cell line. With one of these enzymes, we observed a strong increase in PTM in the presence of Myoclonin-1. This suggests that Myoclonin1 may interact with and modulate the activity of this enzyme. By using luciferase complementation assay and pull down experiments, we could demonstrate that it is indeed the case. Interestingly, the effect is observed even when a DM10 domain alone is co-expressed with the enzyme, suggesting for the first time a role for this domain. In conclusion our data suggest myoclonin-1 modulates specific PTM of MTs. This is of prime importance for microtubule dynamic and notably for neuroblast precursor migration during neocortex development. This could be the mechanism that explains why pathological forms of myoclonin-1 affect brain development.
Research center :
Giga-Neurosciences - ULiège
Disciplines :
Neurology
Author, co-author :
Medard, Laurie ;  Université de Liège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Lakaye, Bernard ;  Université de Liège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Other collaborator :
Godin, Juliette ;  Université de Liège > Giga - Neurosciences
Coumans, Bernard ;  Université de Liège > GIGA - Neurosciences
de Nijs, Laurence;  University of Maastricht > MHeNS,
Language :
English
Title :
Myoclonin 1 modulates the post-translational modification of microtubules
Publication date :
2015
Event name :
BSCDB SPRING MEETING 2015
Event organizer :
Eric Bellefroid (ULB), Laurent Nguyen (ULg) and Eve Seuntjens (ULg)
Event place :
Liège, Belgium
Event date :
5 juin 2015
Name of the research project :
Study of the interaction between EFHC1 and TTLL6, and its functional implication.
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 29 January 2016

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