Reference : Experimental evaluation does not reveal a direct effect of microRNA from the callipyg...
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/189897
Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression.
English
Cheng, Huijun mailto [Université de Liège - ULg > > > Doct. sc. vété. (Bologne)]
Xu, Xuewen [> >]
Hadfield, Tracy [> >]
Cockett, Noelle [> >]
Charlier, Carole mailto [Université de Liège > Département des productions animales (DPA) > GIGA-R : Génomique animale >]
Georges, Michel mailto [Université de Liège > Département des productions animales (DPA) > GIGA-R : Génomique animale >]
Takeda, Haruko mailto [Université de Liège > > GIGA-Research >]
30-Oct-2014
BMC genomics
15
944
Yes (verified by ORBi)
International
1471-2164
1471-2164
England
[en] Animals ; COS Cells ; Cercopithecus aethiops ; Gene Expression Regulation ; Genomic Imprinting ; Intercellular Signaling Peptides and Proteins/metabolism ; MicroRNAs/metabolism ; Muscle, Skeletal/metabolism ; Sheep/genetics ; Transfection
[en] BACKGROUND: Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect. RESULTS: We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo. CONCLUSIONS: This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/189897

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