Article (Scientific journals)
Study of intact virus-like particles of human papillomavirus by capillary electrophoresis
Bettonville, Virginie; Nicol, Jérôme; Thelen, Nicolas et al.
2016In Electrophoresis, 37, p. 579-586
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Keywords :
Capillary electrophoresis; Human papillomavirus; Virus-like particles; Surfactant
Abstract :
[en] Virus-like particles of human papillomavirus (HPV-VLP), resulting from the self-assembly of the capsid proteins (L1 or L1 and L2), have been widely used to study HPV as they are similar to the native virion. Moreover, two prophylactic vaccines, Gardasil® and Cervarix®, are based on HPV-VLP L1. Analytical techniques currently used to characterize HPV-VLP, such as SDS-PAGE, Western blot, ELISA, are time-consuming and semi-quantitative. In this study, capillary electrophoresis (CE) was evaluated for the analysis of intact HPV16-VLP. The usefulness of capillary inner wall coating as well as various BGEs, pH and detergent additives were investigated. Reproducible HPV-VLP analysis in CE was achieved using poly(ethylene oxide) coated capillary and a BGE containing high salt concentration and low SDS concentration. The developed method enables HPV-VLP detection in less than 10 min (migration times RSD : 1.6 %). The identity of HPV-VLP peak was confirmed by comparison with a sample obtained from a wild-type baculovirus and with VLP-based vaccine, Gardasil®, after adjuvant dissolution. Finally, we applied the developed methodology to VLP-based vaccines, demonstrating that CE could be successfully used for vaccine quality control.
Research center :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Bettonville, Virginie ;  Université de Liège > Département de pharmacie > Analyse des médicaments
Nicol, Jérôme ;  Université de Liège > Département des sciences biomédicales et précliniques > Virologie
Thelen, Nicolas  ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire
Thiry, Marc  ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire
Fillet, Marianne ;  Université de Liège > Département de pharmacie > Analyse des médicaments
Jacobs, Nathalie  ;  Université de Liège > Département des sciences biomédicales et précliniques > Virologie
Servais, Anne-Catherine  ;  Université de Liège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Study of intact virus-like particles of human papillomavirus by capillary electrophoresis
Publication date :
2016
Journal title :
Electrophoresis
ISSN :
0173-0835
eISSN :
1522-2683
Publisher :
Vch Publishers, Weinheim, Germany
Volume :
37
Pages :
579-586
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Fonds spéciaux pour la recherche
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
ULiège - Université de Liège [BE]
Fonds Léon Fredericq [BE]
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