Article (Scientific journals)
Characterization of a novel extended-spectrum TEM-type beta-lactamase, TEM-164, in a clinical strain of Klebsiella pneumoniae in Tunisia.
Ben Achour, Nahed; Mercuri, Paola; Ben Moussa, Mohamed et al.
2009In Microbial Drug Resistance: Mechanism, Epidemiology, and Disease, 15 (3), p. 195-9
Peer Reviewed verified by ORBi
 

Files


Full Text
BenAchour1_2009.pdf
Publisher postprint (100.85 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Anti-Bacterial Agents/pharmacology; Conjugation, Genetic; Drug Resistance, Multiple, Bacterial; Humans; Klebsiella Infections/epidemiology/microbiology; Klebsiella pneumoniae/drug effects/enzymology/genetics; Microbial Sensitivity Tests; Molecular Sequence Data; Mutation; Sequence Analysis, DNA; Tunisia/epidemiology; beta-Lactam Resistance; beta-Lactamases/classification/genetics; beta-Lactams/pharmacology
Abstract :
[en] Klebsiella pneumoniae ML1708 exhibited a multiresistance phenotype, including resistance to all beta-lactams tested, chloramphenicol, ciprofloxacin, nalidixic acid, tetracycline, and streptomycin. The double-disk synergy test was positive. ML1708 harbored a 50 kb conjugative plasmid that encoded a beta-lactamase of pI 5.5. The corresponding bla gene was identified by polymerase chain reaction and sequencing as a bla(TEM) gene. The deduced protein sequence revealed a new variant of TEM-1 beta-lactamase designated TEM-164. TEM-164 contains the unusual following mutations: L40V and I279T. These modifications may result in a change of the pI to 5.5 and hydrolyze cefotaxime and ceftazidime.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ben Achour, Nahed
Mercuri, Paola ;  Université de Liège > Département des sciences de la vie > Macromolécules biologiques
Ben Moussa, Mohamed
Galleni, Moreno ;  Université de Liège - ULiège
Belhadj, Omrane
Language :
English
Title :
Characterization of a novel extended-spectrum TEM-type beta-lactamase, TEM-164, in a clinical strain of Klebsiella pneumoniae in Tunisia.
Publication date :
2009
Journal title :
Microbial Drug Resistance: Mechanism, Epidemiology, and Disease
ISSN :
1076-6294
eISSN :
1931-8448
Publisher :
Mary Ann Liebert, Larchmont, United States - New York
Volume :
15
Issue :
3
Pages :
195-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 November 2015

Statistics


Number of views
25 (2 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
10
Scopus citations®
without self-citations
10
OpenCitations
 
6

Bibliography


Similar publications



Contact ORBi