[en] The effects of lactoferrin (LF) on the immune system
have already been shown by many studies. Unfortunately,
the current methods used to measure LF levels
in milk do not permit the study of the genetic variability
of lactoferrin or the performance of routine genetic evaluations.
The first aim of this research was to derive a
calibration equation permitting the prediction of LF in
milk by mid-infrared spectrometry (MIR). The calibration
with partial least squares on 69 samples showed
a ratio of standard error of cross-validation to standard
deviation equal to 1.98. Based on this value, the calibration
equation was used to establish an LF indicator
trait (predicted LF; pLF) on a large number of milk
samples (n = 7,690). A subsequent study of its variability
was conducted, which confirmed that stage of lactation
and lactation number influence the overall pLF
level. Small differences in mean pLF among 7 dairy
breeds were also observed. The pLF content of Jersey
milk was significantly higher than that in Holstein
milk. Therefore, the choice of breed could change the
expected LF level. Heritability estimated for pLF was
19.7%. The genetic and phenotypic correlations between
somatic cell score and pLF were 0.04 and 0.26,
respectively. As somatic cell score increases in presence
of mastitis, this observation seems to indicate that pLF,
or a function of observed pLF, compared with expected
LF might have potential as an indicator of mastitis.
The negative genetic correlation (−0.36) between milk
yield and pLF could indicate an undesirable effect of
selection for high milk production on the overall LF
level.
Disciplines :
Genetics & genetic processes Animal production & animal husbandry
Baker, E. N. 2005. Lactoferrin: A multi-tasking protein par excellence. Cell. Mol. Life Sci. 62:2529-2530.
Baker, E. N., and H. M. Baker. 2005. Molecular structure, binding properties and dynamics of lactoferrin. Cell. Mol. Life Sci. 62:2531-2539.
Baker, H. M., and E. N. Baker. 2004. Lactoferrin and iron: Structural and dynamic aspects of binding and release. Biometals 17:206-216.
Baumrucker, C. R. 2000. Mammary mechanisms for lactoferrin: Interactions with IGFBP-3. Biotechnol. Agron. Soc. Environ. 4:5-12.
Baumrucker, C. R., F. Schanbacher, Y. Shang, and M. H. Green. 2005. Lactoferrin interaction with retinoid signaling: Cell growth and apoptosis in mammary cells. Domest. Anim. Endocrinol. 30:289-303.
Baveye, S., E. Elass, J. Mazurier, G. Spik, and D. Legrand. 1999. Lactoferrin: A multifunctional glycoprotein involved in the modulation of the inflammatory process. Clin. Chem. Lab. Med. 37:281-296.
Brock, J. 1995. Lactoferrin: A multifunctional immunoregulatory protein? Immunol. Today 16:417-419.
Farnaud, S., and R. W. Evans. 2003. Lactoferrin - A multifunctional protein with antimicrobial properties. Mol. Immunol. 40:395-405.
Gaunt, S. N., N. Raffio, E. T. Kingsbury, R. A. Damon, W. H. Johnson, and B. A. Mitchell. 1980. Variation of lactoferrin and mastitis and their heritabilities. J. Dairy Sci. 63:1874-1880.
Gengler, N., G. T. Wiggans, and A. Gillon. 2004. Estimated heterogeneity of phenotypic variance of test-day yield with a structural variance model. J. Dairy Sci. 87:1908-1916.
Hagiwara, S.-I., K. Kawai, A. Anri, and H. Nagahata. 2003. Lactoferrin concentrations in milk from normal and subclinical mastitis cows. J. Vet. Med. Sci. 65:319-323.
Harmon, R. J., F. L. Schanbacher, L. C. Ferguson, and K. L. Smith. 1976. Changes in Lactoferrin, immunoglobulin G, bovine serum albumin, and α-lactalbumin during acute experimental and natural coliform mastitis in cows. Infect. Immun. 13:533-542.
Klobasa, F., B. Senft, F. Meyer, and U. E. Pfleiderer. 1977. Untersuchungen uber Lactoferrin and Immoglobulin G in der Kuhmilch. 2. Mitteilung: die Konzentration von Lactoferrin and Immunoglobulin G in Abhangigkeit von der Laktationsnummer, vom Alter sowie genetische Aspekte dieser Protein. Zuchtungskunde 49:110-119.
Koivula, M., E. A. Mäntysaari, E. Negussie, and T. Serenius. 2005. Genetic and phenotypic relationships among milk yield and somatic cell count before and after clinical mastitis. J. Dairy Sci. 88:827-833.
Kutila, T., L. Suojala, T. Lehtolainen, H. Saloniemi, L. Kaartinen, M. Tähti, K. Seppälä, and S. Pyörälä. 2004. The efficacy of bovine lactoferrin in the treatment of cows with experimentally induced Escherichia coli mastitis. J. Vet. Pharmacol. Therap. 27:197-202.
Lidauer, M., and E. A. Mäntysaari. 1999. Multiple-trait reduced rank random regression test-day model for production traits. Proc. 1999 Interbull Meeting, Zurich, Switzerland. Interbull Bull. 22:74-80.
Lock, A. L., and P. C. Garnsworthy. 2003. Seasonal variation in milk conjugated linoleic acid and Δ9-desaturase activity in dairy cows. Livest. Prod. Sci. 79:47-59.
Martin, A. A., M. A. Faust, L. J. Rowe, and E. J. Lonergan. 2003. Associations of lactoferrin concentrations in milk with indicators of mastitis in dairy cows. J. Dairy Sci. 86(Suppl. 1):129. (Abstr.)
Mead, P. E., and J. W. Tweedie. 1990. cDNA and protein sequence of bovine lactoferrin. Nucleic Acids Res. 18:7167.
Misztal, I. 2007. BLUPF90 family of programs. University of Georgia, Athens, http://nce.ads.uga.edu/~ignacy/numpub/blupf90/ Accessed Jan. 2, 2007.
Othmane, M. H., M. Ben Hamouda, and H. Hammami. 2004. Multivariate animal model estimates of genetic, environmental and phenotypic correlations for early lactation milk yield and composition in Tunisian Holstein-Friesians. Proc. 2004 Interbull Meeting, Sousse, Tunisia. Interbull Bull. 32:129-132.
Pierce, A., D. Colavizza, M. Benaissa, P. Maes, A. Tartar, J. Montreuil, and G. Spik. 1991. Molecular cloning and sequence analysis of bovine lactotransferrin. Eur. J. Biochem. 196:177-184.
Pugovel, G., C. R. Baumrucker, H. Sauerwein, R. Rühl, E. Ontsouka, H. M. Hammon, and J. W. Blum. 2005. Effects of an enhanced vitamin A intake during the dry period on retinoids, lactoferrin, IGF system, mammary gland epithelial cell apoptosis, and subsequent lactation in dairy cows. J. Dairy Sci. 88:1785-1800.
Roman, R. M., and C. J. Wilcox. 2000. Bivariate animal model estimates of genetic, phenotypic, and environmental correlations for production, reproduction, and somatic cells in Jerseys. J. Dairy Sci. 83:829-835.
Sinnaeve, G., P. Dardenne, R. Agneessens, and R. Biston. 1994. The use of near infrared spectroscopy for the analysis of fresh grass silage. J. Near Infrared Spectrosc. 2:79-84.
Soyeurt, H., P. Dardenne, G. Lognay, D. Veselko, P. Mayeres, and N. Gengler. 2006. Estimating fatty acid content in cow milk using mid-infrared spectrometry. J. Dairy Sci. 89:3690-3695.
Tsuji, S., Y. Hirata, and F. Mukai. 1990. Comparison of lactoferrin content in colostrum between different cattle breeds. J. Dairy Sci. 73:125-128.
Turner, S.-A., and N. A. Thomson. 2006. Lactoferrin in bovine milk is not affected by strain of cow or level of feed. 7th Int. Conf. Lactoferrin, Canada. 84(3):393. (Abstr.)
Veerkamp, R. F., and M. E. Goddard. 1998. Covariance functions across herd production levels for test day records on milk, fat and protein yields. J. Dairy Sci. 81:1690-1701.
Ward, P. P., E. Paz, and O. M. Conneely. 2005. Multifunctional roles of lactoferrin: A critical overview. Cell. Mol. Life Sci. 62:2540-2548.
William, P., and K. Norris. 2001. Near-infrared technology in the agricultural and food industries. American Association of Cereal Chemists, St. Paul, MN.