Hepcidin determination in dried blood by microfluidic LC-MS/MS: comparison of DBS and volumetric absorptive microsampling for matrix effect and recovery.
[en] BACKGROUND: Dried blood analysis experiences a growing interest due to practical, ethical and financial advantages compared with classical wet plasma or serum analysis. Besides classical DBS, new alternatives are commercialized as volumetric absorptive microsampling (VAMS) that are expected to overcome hematocrit influence. RESULTS: The feasibility of hepcidin (a peptide hormone) extraction and determination from DBS and VAMS blood sampling was investigated. Experimental design was used to determine the optimal extraction conditions. Matrix effect and extraction recovery were studied and a special attention was paid to phospholipid removal. CONCLUSION: The data suggest that the combination of VAMS and phospholipid removal plates provides low matrix effect and high sensitivity, and constitutes an easy and promising protocol for hepcidin analysis.
Research center :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Houbart, Virginie ; Université de Liège > Département de pharmacie > Analyse des médicaments
Servais, Anne-Catherine ; Université de Liège > Département de pharmacie > Analyse des médicaments
Napp, Aurore ; Université de Liège > Département de pharmacie > Analyse des médicaments
Merville, Marie-Paule ; Université de Liège > Département de pharmacie > Chimie médicale
Fillet, Marianne ; Université de Liège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Hepcidin determination in dried blood by microfluidic LC-MS/MS: comparison of DBS and volumetric absorptive microsampling for matrix effect and recovery.
Publication date :
2015
Journal title :
Bioanalysis
ISSN :
1757-6180
eISSN :
1757-6199
Publisher :
Future Science, United Kingdom
Volume :
7
Issue :
21
Pages :
2789-99
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE] Fonds Léon Fredericq [BE]
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