Article (Scientific journals)
A Survey of the Kinetic Parameters of Class C Beta-Lactamases. Penicillins
Galleni, Moreno; Frère, Jean-Marie
1988In Biochemical Journal, 255 (1), p. 119-22
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Abstract :
[en] The interaction between six class C beta-lactamases and various penicillins has been studied. All the enzymes behaved in a very uniform manner. Benzylpenicillin exhibited relatively low kcat. values (14-75 s-1) but low values of Km resulted in high catalytic efficiencies [kcat./Km = 10 X 10(6)-75 X 10(6) M-1.s-1]. The kcat. values for ampicillin were 10-100-fold lower. Carbenicillin, oxacillin cloxacillin and methicillin were very poor substrates, exhibiting kcat. values between 1 x 10(-3) and 0.1 s-1. The Km values were correspondingly small. It could safely be hypothesized that, with all the tested substrates, deacylation was rate-limiting, resulting in acyl-enzyme accumulation.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Frère, Jean-Marie ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Language :
English
Title :
A Survey of the Kinetic Parameters of Class C Beta-Lactamases. Penicillins
Publication date :
01 October 1988
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, United Kingdom
Volume :
255
Issue :
1
Pages :
119-22
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 November 2015

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