Article (Scientific journals)
Biochemical characterisation of the CTX-M-14 beta-lactamase
Ishii, Y.; Galleni, Moreno; Ma, L. et al.
2007In International Journal of Antimicrobial Agents, 29 (2), p. 159-164
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Keywords :
CTX-M-14; extended-spectrum beta-lactamase; antibiotic resistance; kinetic study
Abstract :
[en] Cefotaxime-resistant Escherichia coli TUM 1121 was isolated from an abscess of an 83-year-old patient. The CTX-M-14 gene was located on a 70 kb plasmid. The enzyme was purified and its activity was analysed. CTX-M-14 was poorly active against ceftazidime and aztreonam. Aztreonam behaved as a competitive inhibitor. Among the tested suicide substrates for class A beta-lactamases, sulbactam was a rather good substrate. Tazobactam and clavulanic acid behaved as inactivators. The interactions between clavulanic acid and CTX-M-14 were characterised by progressive inactivation of the P-lactamase. Carbapenems such as imipenem, meropenem or doripenem did not behave as inactivators of CTX-M-14, however very small k(cat) values were observed. This result shows that CTX-M-14 is able to hydrolyse carbapenems. (c) 2006 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Disciplines :
Pharmacy, pharmacology & toxicology
Microbiology
Immunology & infectious disease
Author, co-author :
Ishii, Y.
Galleni, Moreno ;  Université de Liège - ULiège > Département des sciences de la vie > Macromolécules biologiques
Ma, L.
Frère, Jean-Marie ;  Université de Liège - ULiège > Département des sciences de la vie > Département des sciences de la vie
Yamaguchi, K.
Language :
English
Title :
Biochemical characterisation of the CTX-M-14 beta-lactamase
Publication date :
February 2007
Journal title :
International Journal of Antimicrobial Agents
ISSN :
0924-8579
eISSN :
1872-7913
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
29
Issue :
2
Pages :
159-164
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 November 2015

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