Article (Scientific journals)
A facile and efficient strategy to enhance hydrophilicity of zwitterionic sulfoalkylbetaine type monoliths.
Yuan, Guangxin; Peng, Yongbo; Liu, Zhenghua et al.
2013In Journal of Chromatography. A, 1301, p. 88-97
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Keywords :
Acetonitriles/chemistry; Acrylamides/chemistry; Allantoin/chemistry/isolation & purification; Betaine/analogs & derivatives/chemistry; Chromatography, Liquid/instrumentation/methods; Formates/chemistry; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Models, Chemical; Peptides/chemistry/isolation & purification; Phenols/chemistry/isolation & purification; Polymerization; Static Electricity; Structure-Activity Relationship; Urea/chemistry/isolation & purification; Electrostatic repulsion; Hydrophilic interaction chromatography; Monolithic column; N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine; Zwitterionic
Abstract :
[en] In order to prepare zwitterionic HILIC monolithic columns with high polarity, the highly hydrophilic monomer N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine (SPDA) and crosslinker N,N'-methylenebisacrylamide (MBA) were employed for developing a novel sulfoalkylbetaine type stationary phase. The polymerization parameters were systematically optimized in order to obtain a satisfactory performance for column permeability, mechanical stability, hydrophilicity, efficiency and selectivity. Compared to the previously reported poly(N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl)ammonium betaine-co-ethylene dimethacrylate) (poly(SPE-co-EDMA)) monolith and the poly(SPDA-co-EDMA) monolith that we developed, a significantly enhanced hydrophilicity was obtained on the poly(SPDA-co-MBA) monolithic column, illustrated by the lowered critical composition of the mobile phase corresponding to the transition from the HILIC to the RP mode. Excellent permeability, reproducibility and stability were achieved on this optimized poly(SPDA-co-MBA) monolith. A column efficiency of 70,000plates/m was obtained for the analysis of bases at a linear velocity of 1.95mm/s. As expected, by studying the influence of mobile phase pH and salt concentration on their retention, a weak electrostatic repulsion interaction for negatively charged analytes was also observed at low organic solvent content on the poly(SPDA-co-MBA) monolithic column. The final optimized poly(SPDA-co-MBA) monolith exhibited good selectivity for a series of polar compounds, such as phenols, bases, benzoic acid derivatives, small peptides, urea and allantoin.
Research center :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Yuan, Guangxin
Peng, Yongbo
Liu, Zhenghua
Hong, Jieya
Xiao, Yao
Guo, Jialiang
Smith, Norman W.
Crommen, Jacques ;  Université de Liège > Département de pharmacie > Département de pharmacie
Jiang, Zhengjin
Language :
English
Title :
A facile and efficient strategy to enhance hydrophilicity of zwitterionic sulfoalkylbetaine type monoliths.
Publication date :
2013
Journal title :
Journal of Chromatography. A
ISSN :
0021-9673
eISSN :
1873-3778
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
1301
Pages :
88-97
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2013 Elsevier B.V. All rights reserved.
Available on ORBi :
since 23 November 2015

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