[en] Understanding the molecular parameters that regulate cross-species transmission and host
adaptation of potential pathogens is crucial to control emerging infectious disease. Although
microbial pathotype diversity is conventionally associated with gene gain or loss, the role of
pathoadaptive nonsynonymous single-nucleotide polymorphisms (nsSNPs) has not been
systematically evaluated. Here, our genome-wide analysis of core genes within Salmonella
enterica serovar Typhimurium genomes reveals a high degree of allelic variation in
surface-exposed molecules, including adhesins that promote host colonization. Subsequent
multinomial logistic regression, MultiPhen and Random Forest analyses of known/suspected
adhesins from 580 independent Typhimurium isolates identifies distinct host-specific nsSNP
signatures. Moreover, population and functional analyses of host-associated nsSNPs for
FimH, the type 1 fimbrial adhesin, highlights the role of key allelic residues in host-specific
adherence in vitro. Together, our data provide the first concrete evidence that functional
differences between allelic variants of bacterial proteins likely contribute to pathoadaption to
diverse hosts.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Yue, Min; University of Pennsylvania - Penn > Pathobiology
Han, Xiangan; University of Pennsylvania - Penn > Pathobiology
De Masi, Leon; University of Pennsylvania - Penn > Pathobiology
Zhu, Chunhong; University of Pennsylvania - Penn > Pathobiology
Ma, Xun; University of Pennsylvania - Penn > Pathobiology
Zhang, Junjie; University of Pennsylvania - Penn > Pathobiology
Wu, Renwei; University of Pennsylvania - Penn > Pathobiology
Schmieder, Robert; San Diego State University > Computer Science
Kaushik, Radhey; South Dakota State University > Veterinary and Biomedical Science
Fraser, George; Pennesylvania Department of Health
Zhao, Shaohua; Center for Veterinary Medicine, Maryland
McDermott, Patrick; Center for Veterinary Medicine, Maryland > Animal and Food Microbiology
Weill, François-Xavier; Institut Pasteur, Paris > Unité des Bactéries Pathogènes Entériques
Mainil, Jacques ; Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Bactériologie et pathologie des maladies bactériennes
Arze, Cesar; University of Maryland at Baltimore - UMB > Microbiology and Immmunology
Fricke, W. Florian; University of Maryland at Baltimore - UMB > Microbiology and Immunology
Edwards, Robert; Argonne National Laboratory > Mathematics and Computer Science
Brisson, Dustin; University of Pennsylvania - Penn > Biology
zhang, Nancy; University of Pennsylvania - Penn > Statistics
Rankin, Shelley; University of Pennsylvania - Penn > Pathobiology
Schifferli, Dieter; University of Pennsylvania - Penn > Pathobiology
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