HPLC-DAD IDENTIFICATION OF SOME FLAVONOIDS FROM THE LEAVES AND AERIAL PARTS OF BRYONIA ALBA L. SPECIES SPONTANEOUS IN THE ROMANIAN FLORA

Bryonia alba L. is a climbing species, spontaneous in the Romanian flora, which can be found throughout the whole country [1]. It is known for its cytotoxic, analgesic, antipyretic, anti-inflammatory, antibacterial, anti-rheumatic, laxative-purgative and smooth muscle relaxant proprieties, being used both in traditional medicine and in homeopathy [2]. The main objective of this study consists in the evaluation of the flavonoid profile of this species.
The vegetal material was harvested from the spontaneous flora of Cluj county (Romania). The vegetal extracts were obtained by ultrasonication, in methanol. Analysis of flavonoids was performed by a HPLC-DAD method and revealed mainly the presence of C-glycosides, of which saponarine was found as the main compound. Quantification of saponarin was also performed, using the HPLC method, on samples collected at different periods of time. Variation of the quantity of saponarine according to harvested samples was determined.
Further analysis are under process in order to investigate the structure of these flavonoids and the pharmacological effects of the Bryonia alba L. plant extracts.

References:

1. *** Flora Europea, vol. 2, Cambridge, Univ. Press. Cambridge London-New York Melbourne, 1979, p. 298-299
2. Demarque D, Jouanny J, Poitevin B, Saint Jean Y. Pharmacologie et matière médicale homéopathique, 3ième edition, France, CEDH, 2007.