Reference : Validation of a method for the determination of sterols and triterpenes in the aerial pa...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/18778
Validation of a method for the determination of sterols and triterpenes in the aerial part of Justicia anselliana (Nees) T. Anders by capillary gas chromatography.
English
Kpoviessi, Dossou Sika Salome [> > > >]
Gbaguidi, Fernand [> > > >]
Gbenou, Joachim [> > > >]
Accrombessi, Georges [> > > >]
Moudachirou, Mansourou [> > > >]
Rozet, Eric mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Hubert, Philippe mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Quetin-Leclercq, Joelle [> > > >]
2008
Journal of Pharmaceutical & Biomedical Analysis
Elsevier Science
48
4
1127-35
Yes (verified by ORBi)
International
0731-7085
Oxford
United Kingdom
[en] Acanthaceae/chemistry ; Calibration ; Cholesterol/analogs & derivatives/analysis/chemistry ; Chromatography, Gas/methods ; Molecular Structure ; Phytosterols/analysis/chemistry ; Plant Components, Aerial/chemistry ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity ; Sitosterols/analysis/chemistry ; Solid Phase Extraction ; Sterols/analysis/chemistry ; Stigmasterol/analysis/chemistry ; Triterpenes/analysis/chemistry
[en] An accurate and sensitive method, combining soxhlet extraction, solid phase-extraction and capillary gas chromatography is described for the quantitative determination of one triterpene (lupeol) and three sterols (stigmasterol, campesterol and beta-sitosterol) and the detection of another triterpene (alpha-amyrin) from the aerial part of Justicia anselliana. This is the first method allowing the quantification of sterols and triterpenes in this plant. It has been fully validated in order to be able to compare the sterol and triterpene composition of different samples of J. anselliana and therefore help to explain the allelopathic activity due to these compounds. This method showed that the aerial part of J. anselliana contained (292+/-2)mg/kg of lupeol, (206+/-1)mg/kg of stigmasterol, (266+/-2)mg/kg of campesterol and (184+/-9)mg/kg of beta-sitosterol.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/18778
10.1016/j.jpba.2008.08.036

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
Publié_sdarticle.pdfPublisher postprint1.1 MBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.