Validation of a method for the determination of sterols and triterpenes in the aerial part of Justicia anselliana (Nees) T. Anders by capillary gas chromatography.
[en] An accurate and sensitive method, combining soxhlet extraction, solid phase-extraction and capillary gas chromatography is described for the quantitative determination of one triterpene (lupeol) and three sterols (stigmasterol, campesterol and beta-sitosterol) and the detection of another triterpene (alpha-amyrin) from the aerial part of Justicia anselliana. This is the first method allowing the quantification of sterols and triterpenes in this plant. It has been fully validated in order to be able to compare the sterol and triterpene composition of different samples of J. anselliana and therefore help to explain the allelopathic activity due to these compounds. This method showed that the aerial part of J. anselliana contained (292+/-2)mg/kg of lupeol, (206+/-1)mg/kg of stigmasterol, (266+/-2)mg/kg of campesterol and (184+/-9)mg/kg of beta-sitosterol.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Kpoviessi, Dossou Sika Salome
Gbaguidi, Fernand
Gbenou, Joachim
Accrombessi, Georges
Moudachirou, Mansourou
Rozet, Eric ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Hubert, Philippe ; Université de Liège - ULiège > Département de pharmacie > Chimie analytique
Quetin-Leclercq, Joelle
Language :
English
Title :
Validation of a method for the determination of sterols and triterpenes in the aerial part of Justicia anselliana (Nees) T. Anders by capillary gas chromatography.
Hutchinson F.R.D., and Dalziel J.M. Flora of West tropical Africa (1963), Witstable Litho, Printers Ltd., London pp. 391-427
S. De Souza, Flore du Bénin. Catalogue des plantes du Bénin, Tome 1, imprimerie Notre-Dame, Cotonou, 1987, p 2.
Watt J.M., and Breyer-brandwikj M.G. The Medicinal and Poisonous Plants of Southern and Eastern Africa. second ed. (1962), Livingstone, London
Kossou D.K., Gbèhounou G., Ahanchédé A., Ahohuendo B., Bouraïma Y., and VanHuis A. Insect Sci. Appl. 21 (2001) 123-132
Rice E.L. Allelopathy (1984), Academic Press, New York
Torres A., Olivia R.M., Castellano D., and Cross P. Proceedings of the First World Congress on Allelopathy: A Science of the Future. SAI (Universyti Cadiz), Spring Cadiz (1996)
Ahanchede A., Gbehounou G., Kossou K.D., Yayi E., and Akpo E. Ann. FSA 6 (2004). http://www.annales-fsa.bj.refer.org
Kpoviessi D.S.S., Gbénou J.D., Gbaguidi F.A., Haddad M., Accrombessi G.C., Moudachirou M., and Quetin-Leclercq J. EJNS I (2006) 12-19. http://www.ejns.univ-lyon1.fr
Takagi T., and Iida T. JAOCS Octobre (1980) 326-330
Bianchini J.-P., and Gaydou E.M. J. Chromatogr. 329 (1985) 231-246
Willuhn G., and May S. Planta Med. 46 (1982) 153-158
Biesboer D.D., D'Amour P., Wilson S.R., and Mahlberg P. Phytochemistry 21 (1982) 1115-1118
Palmer M.A., and Bowden B.N. Phytochemistry 16 (1977) 459-463
Lagarda M.J., Garcia-Llatas G., and Farré R. J. Pharm. Biomed. Anal. 41 (2006) 1486-1496
Hubert Ph., Chiap P., Crommen J., Boulanger B., Chapuzet E., Mercier N., Bervoas-Martin S., Chevalier P., Grandjean D., Lagorce P., Lallier M., Laparra M.C., Laurentie M., and Nivet J.C. Anal. Chim. Acta 391 (1999) 135-148
Chiap P., Hubert Ph., Boulanger B., and Crommen J. Anal. Chim. Acta 391 (1999) 227-238
Hubert Ph., Nguyen-Huu J.J., Boulanger B., Chapuzet E., Chiap P., Cohen N., Compagnon P.A., Dewé W., Feinberg M., Lallier M., Laurentie M., Mercier N., Muzard G., Nivet C., and Valat L. STP Pharma Prat. 13 (2003) 101-138
Hubert Ph., Nguyen-Huu J.-J., Boulanger B., Chapuzet E., Chiap P., Cohen N., Compagnon P.-A., Dewe W., Feinberg M., Lallier M., Laurentie M., Mercier N., Muzard G., Nivet C., and Valat L. J. Pharm. Biomed. Anal. 36 (2004) 579-586
Block S., Brkic D., Hubert P., and Quetin-Leclerq J. Phytochem. Anal. 16 (2005) 342-348
Miller J.C., and Miller J.N. Statistics for Analytical Chemistry. 5th ed. (2000), Ellis Horwood, New York
Hubert Ph., Nguyen-Huu J.-J., Boulanger B., Chapuzet E., Chiap P., Cohen N., Compagnon P.A., Dewe W., Feinberg M., Lallier M., Laurentie M., Mercier N., Muzard G., Nivet C., and Valat L. J. Pharm. Biomed. Anal. 45 (2007) 70-81
Hubert Ph., Nguyen-Huu J.J., Boulanger B., Chapuzet E., Cohen N., Compagnon P.A., Dewe W., Feinberg M., Laurentie M., Mercier N., Muzard G., Nivet C., and Valat L. J. Pharm. Biomed. Anal. 45 (2007) 82-96
Rozet E., Ceccato A., Hubert C., Ziemons E., Oprean R., Rudaz S., Boulanger B., and Hubert Ph. J. Chromatogr. A 1158 (2007) 111-125
