MHC multimers; disease- and therapy-induced T cell immunity; melanoma patients
Abstract :
[en] Abstract
The use of fluorescently labeled MHC multimers has become an essential technique for the analysis of disease- and therapy-induced T cell immunity. While classical MHC multimer analyses are well-suited for the detection of immune responses against a few epitopes, limitations on patient sample size preclude a comprehensive analysis of T cell immunity.
To address this issue, we have developed a combinatorial encoding strategy that allows the parallel detection of a multitude of different T cell populations within a single sample. Detection of antigen-specific T cells from peripheral blood by combinatorial encoding is as efficient as detection with conventional PE labeled multimers, but results in a significantly increased sensitivity, and most importantly, allows comprehensive screens to be performed on patient material. Proof of principle for the feasibility of large-scale screening of patient material was obtained by analysis of HLA-A3 restricted T cell responses against known and potential melanoma-associated antigens in peripheral blood from melanoma patients.
2
Disciplines :
Hematology
Author, co-author :
Reker Hadrup, Sine
Bakker, Arnold H
Shu, Chengyi J.
Andersen, Rikke S.
van Veluw, Jerre
Hombrink, Pleun
Castermans, Emilie ; Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Straten, Per thor
Blank, Christian
Haanen, John B.
Heemskerk, Mirjam H.
Schumacher, Ton N.
Language :
English
Title :
Parallel detection of antigen-specific T-cell responses by multidimensional encoding of peptide-Major Histocompatibility Complexes
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