[en] In order to investigate the effects of the crosslinker on the separation performance of polar zwitterionic sulfoalkylbetaine-type monolithic columns, three crosslinkers, i.e. 1,4-bis(acryloyl)piperazine (PDA), ethylene dimethacrylate (EDMA) and N,N'-methylenebisacrylamide (MBA), were copolymerized with the hydrophilic monomer N,N-dimethyl-N-acryloyloxyethyl-N-(3-sulfopropyl)ammonium betaine (SPDA). The chromatographic properties of the three hydrophilic sulfoalkylbetaine-type monolithic columns, including column efficiency, permeability, porosity and separation mechanism, were systematically compared using scanning electron microscopy or micro-HPLC. Good selectivity in micro-HPLC separations was achieved on all three monolithic columns. The results indicate that the polarity of sulfoalkylbetaine-type monolithic columns may be related to the polarity of the crosslinker, which further affects column selectivity and efficiency. A particularly high column efficiency (100,000 plates/m) was obtained on the novel poly(SPDA-co-PDA) monolithic column at a linear velocity of 1mm/s using thiourea as test analyte. A higher resolution was also observed for nucleobases, nucleosides and hydrophilic organic acids on this novel poly(SPDA-co-PDA) monolithic column compared to the other two columns.
Research center :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Liu, Chusheng
Chen, Weijia
Yuan, Guangxin
Xiao, Yao
Crommen, Jacques ; Université de Liège > Département de pharmacie > Département de pharmacie
Xu, Shihai
Jiang, Zhengjin
Language :
English
Title :
Influence of the crosslinker type on the chromatographic properties of hydrophilic sulfoalkylbetaine-type monolithic columns.
Publication date :
2014
Journal title :
Journal of Chromatography. A
ISSN :
0021-9673
eISSN :
1873-3778
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
1373
Pages :
73-80
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2014 Elsevier B.V. All rights reserved.
Alpert A.J. Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds. J. Chromatogr. 1990, 499:177-196.
Hosoya K., Kishii Y., Kimata K., Araki T., Tanaka N. Efficiency of porous hydrophilic polymer-based packing materials in chromatographic analysis of drugs existing with polypeptide. Chromatographia 1994, 38:177-182.
Wu M., Wu R., Wang F., Ren L., Dong J., Liu Z., Zou H. One-pot process for fabrication of organic-silica hybrid monolithic capillary columns using organic monomer and alkoxysilane. Anal. Chem. 2009, 81:3529-3536.
Underberg-Chitoe U.K., Underberg W.J.M., Lingeman H. Liquid chromatography of basic solutes on silica gel and aluminum oxide. Anal. Sci. 1988, 4:91-96.
Chen M., Ma Q., Feng Y.Q. A bidentate amino stationary phase for hydrophilic interaction chromatography. J. Liq. Chromatogr. Related Technol. 2009, 32:2164-2175.
Jandera P. Stationary and mobile phases in hydrophilic interaction chromatography: a review. Anal. Chim. Acta 2011, 692:1-25.
Guo Y., Gaiki S. Retention and selectivity of stationary phases for hydrophilic interaction chromatography. J. Chromatogr. A 2011, 1218:5920-5938.
Greco G., Grosse S., Letzel T. Study of the retention behavior in zwitterionic hydrophilic interaction chromatography of isomeric hydroxy- and aminobenzoic acids. J. Chromatogr. A 2012, 1235:60-67.
Puente M.L.D.L., Soto-Yarritu P.L., Anta C. Placing supercritical fluid chromatography one step ahead of reversed-phase high performance liquid chromatography in the achiral purification arena: a hydrophilic interaction chromatography cross-linked diol chemistry as a new generic stationary phase. J. Chromatogr. A 2012, 1250:172-181.
Wang C., Jiang C., Armstrong D.W. Considerations on HILIC and polar organic solvent-based separations: use of cyclodextrin and macrocyclic glycopetide stationary phases. J. Sep. Sci. 2008, 31:1980-1990.
Johnsen E., Wilson S.R., Odsbu I., Krapp A., Malerod H., Skarstad K., Lundanes E. Hydrophilic interaction chromatography of nucleoside triphosphates with temperature as a separation parameter. J. Chromatogr. A 2011, 1218:5981-5986.
Lei W., Zhang L., Wan L., Shi B., Wang Y., Zhang W. Hybrid monolithic columns with nanoparticles incorporated for capillary electrochromatography. J. Chromatogr. A 2012, 1239:64-71.
André C., Guillaume Y.C. Boron nitride nanotubes and their functionalization via quinuclidine-3-thiol with gold nanoparticles for the development and enhancement of the HPLC performance of HPLC monolithic columns. Talanta 2012, 93:274-278.
Jiang Z., Smith N.W., Liu Z. Preparation and application of hydrophilic monolithic columns. J. Chromatogr. A 2011, 1218:2350-2361.
Urban J., Jandera P. Recent advances in the design of organic polymer monoliths for reversed-phase and hydrophilic interaction chromatography separations of small molecules. Anal. Bioanal. Chem. 2013, 405:2123-2131.
Jiang Z., Smith N.W., Ferguson P.D., Taylor M.R. Hydrophilic interaction chromatography using methacrylate-based monolithic capillary column for the separation of polar analytes. Anal. Chem. 2007, 79:1243-1250.
Jiang Z., Reilly J., Everatt B., Smith N.W. Novel zwitterionic polyphosphorylcholine monolithic column for hydrophilic interaction chromatography. J. Chromatogr. A 2009, 1216:2439-2448.
Viklund C., Irgum K. Synthesis of porous zwitterionic sulfobetaine monoliths and characterization of their interaction with proteins. Macromolecules 2000, 33:2539-2544.
Jiang Z., Smith N.W., Ferguson P.D., Taylor M.R. Novel highly hydrophilic zwitterionic monolithic column for hydrophilic interaction chromatography. J. Sep. Sci. 2009, 32:2544-2555.
Lin J., Lin J., Lin X., Xie Z. Preparation of a mixed-mode hydrophilic interaction/anion-exchange polymeric monolithic stationary phase for capillary liquid chromatography of polar analytes. J. Chromatogr. A 2009, 1216:801-806.
Liu Z., Peng Y., Wang T., Yuan G., Zhang Q., Guo J., Jiang Z. Preparation and application of novel zwitterionic monolithic column for hydrophilic interaction chromatography. J. Sep. Sci. 2013, 36:262-269.
Yuan G., Peng Y., Liu Z., Hong J., Xiao Y., Guo J., Smith N.W., Crommen J., Jiang Z. A facile and efficient strategy to enhance hydrophilicity of zwitterionic sulfoalkylbetaine type monoliths. J. Chromatogr. A 2013, 1301:88-97.
Abdurrahmanoglu S., Okay O. Preparation of homogeneous hydrogels by controlling the crosslinker reactivity and availability. J. Macromol. Sci. Pure 2008, 45:769-775.
Wang X., Lin X., Xie Z. Preparation and evaluation of a sulfoalkylbetaine-based zwitterionic monolithic column for CEC of polar analytes. Electrophoresis 2009, 30:2702-2710.
Murfin D.L., Hayashi K., Miller L.E. N,N'-(disubstitutedmethylene) bisacrylamides: preparation and polymerization. J. Polym. Sci. Polym. Chem. 1970, 8:1967-1980.
SciFinder Chemical Abstracts Service 2013, SciFinder, https://scifinder.cas.org
Hoegger D., Freitag R. Investigation of mixed-mode monolithic stationary phases for the analysis of charged amino acids and peptides by capillary electrochromatography. J. Chromatogr. A 2003, 1004:195-208.
Hoegger D., Freitag R. Investigation of conditions allowing the synthesis of acrylamide-based monolithic microcolumns for capillary electrochromatography and of factors determining the retention of aromatic compounds on these stationary phases. Electrophoresis 2003, 24:2958-2972.
Staňková M., Jandera P., Škeříková V., Urban J. Cross-linker effects on the separation efficiency on (poly)methacrylate capillary monolithic columns. Part II. Aqueous normal-phase liquid chromatography. J. Chromatogr. A 2013, 1289:47-57.
Bristow P.A., Knox J.H. Standardization of test conditions for high performance liquid chromatography columns. Chromatographia 1977, 10:279-289.
Wode H., Seidel W.Ber. Precise viscosity measurement of binary liquid mixtures of acetonitrile-water and 1,3-dimethyl-1-imidazolidinone-water. Bunsen Phys. Chem. 2004, 98:927-934.
Fischer J., Jandera P. Chromatographic behaviour of phenylurea pesticides in high-performance liquid chromatography with nitrile- and amino-bonded stationary phases. J. Chromatogr. A 1994, 684:77-92.
Pesek J.J., Matyska M.T., Larrabee S. HPLC retention behavior on hydride-based stationary phases. J. Sep. Sci. 2007, 30:637-647.
Dean J.A. Lange's Handbook of Chemistry 1999, McGraw-Hill, New York, NY. 15th ed.
Alpert A.J. Electrostatic repulsion hydrophilic interaction chromatography for isocratic separation of charged solutes and selective isolation of phosphopeptides. Anal. Chem. 2008, 80:62-76.
Lindner H., Sarg B., Helliger W. Application of hydrophilic-interaction liquid chromatography to the separation of phosphorylated H1 histones. J. Chromatogr. A 1997, 782:55-62.
