Article (Scientific journals)
High reactive oxygen species in fibrotic and non-fibrotic skin of Patients with diffuse cutaneous systemic sclerosis
BOURJI, Khalil; MEYER, Alain; CHATELUS, Emmanuel et al.
2015In Free Radical Biology and Medicine, 87, p. 282-289
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Keywords :
Oxidative stress; ROS; Redox imbalance biomarkers; Skin; Systemic sclerosis; Scleroderma
Abstract :
[en] Systemic Sclerosis (SSc) is a chronic multisystemic connective tissue disease characterized by progressive fibrosis affecting skin and internal organs. Despite serious efforts to unveil the pathogenic mechanisms of SSc, they are still unclear. High levels of Reactive Oxygen Species (ROS) in affected patients have been shown, and ROS are suggested to play a role in fibrosis pathogenesis. In this study we evaluate ROS levels in non-fibrotic and fibrotic skin of patients with SSc and we compare them with those obtained from healthy controls. Patients and Methods We enrolled 9 SSc patients fulfilling the EULAR/ACR classification criteria and 7 healthy controls. Patients included were 4 men and 5 women with mean age of 46 ±10 yrs. Controls were matched by sex and age. All patients were affected by diffuse cutaneous form of SSc and the ANA pattern anti-Scl70. Mean disease duration was 7.5±5 yrs. Skin involvement was evaluated by modified Rodnan Skin Score (mRSS). Skin samples (4 mm punch biopsy) were taken from fibrotic skin and non-fibrotic skin of patients and from healthy controls as well. To detect ROS, specimens were analyzed immediately after sampling by electron paramagnetic resonance spectroscopy. Blood samples have been drawn from all patients and controls to assess oxidative stress biomarkers. Results ROS levels (expressed as median and range, unit of measurement was nmol/l/min/mg of dry weight) were 24.7 (10.9– 47.0) in fibrotic skin, 18.7 (7.3–34.0) in non-fibrotic skin and 7.7 (3.5–13.6) in healthy controls skin. ROS levels in Fibrotic and Non-fibrotic skin of SSc patients were significantly higher than in Healthy Controls (p=0.002 and p=0.009, respectively). ROS levels in fibrotic skin were raised in comparison to non-fibrotic skin, when samples related to each patient were compared (p=0.01). ROS levels in fibrotic skin were correlated with forced vital capacity (r= -0.75, p=0.02) and erythrocyte sedimentation rate (r=0.70, p=0.04). All other clinical and lab parameters showed no significant correlation. When compared to controls, blood from SSc patients showed lower ascorbate (vitamin C) levels (8 [3.8-9.8] vs. 10.5 [9-19.1] mg/L, p=0.004) and higher lipid peroxides (873.5 [342-1973] vs. 422 [105-576] μmol/L, p=0.004). Conclusion Our results indicate the presence of high oxidative stress both in non-fibrotic skin and fibrotic skin of SSc patients, but with higher tendency in the latter. Raised ROS levels in non-fibrotic skin of SSc patients might be a hint of early involvement in skin fibrogenesis. However, a longitudinal prospective study is necessary for such proof.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
BOURJI, Khalil;  Università degli Studi di Padova > Reumatology Unit
MEYER, Alain;  Université Louis Pasteur (Strasbourg) - ULP > Division of Reumatology > Physiology and Functional Explorations
CHATELUS, Emmanuel;  Université Louis Pasteur (Strasbourg) - ULP > Division of Rheumatology
PINCEMAIL, Joël ;  Centre Hospitalier Universitaire de Liège - CHU > Service de Chirurgie cardio-vasculaire et thoracique
PIGATTO, Erika;  Università degli Studi di Padova > Rheumatology Unit
Defraigne, Jean-Olivier ;  Université de Liège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
SINGH, François;  Université Louis Pasteur (Strasbourg) - ULP > Division of Physiology and Functional Explorations
Charlier, Corinne  ;  Université de Liège > Département de pharmacie > Chimie toxicologique
GENY, Bernard;  Université Louis Pasteur (Strasbourg) - ULP > Division of Physiology and Functional Explorations
GOTTENBERG, Jacques-Eric;  Université Louis Pasteur (Strasbourg) - ULP > Division of Rheumatology
PUNZI, Leonardo;  Università degli Studi di Padova > Rheumatology Unit
COZZI, Franco;  Università degli Studi di Padova > Rheumatology Unit
SIBILIA, Jean;  Université Louis Pasteur (Strasbourg) - ULP > Division of Rheumatology
More authors (3 more) Less
Language :
English
Title :
High reactive oxygen species in fibrotic and non-fibrotic skin of Patients with diffuse cutaneous systemic sclerosis
Publication date :
October 2015
Journal title :
Free Radical Biology and Medicine
ISSN :
0891-5849
eISSN :
1873-4596
Publisher :
Elsevier Science, Tarrytown, United States - New York
Volume :
87
Pages :
282-289
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 October 2015

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