Article (Scientific journals)
A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals
Brenez, C.; Gerkens, P.; Mazzucchelli, Gabriel et al.
2004In Talanta, 63 (5), p. 1225-1230
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Keywords :
PCDD/F mixture; seal PBMC; lymphoproliferation; proteomics
Abstract :
[en] The cell lines chosen have demonstrated a positive response in terms of cell proliferation and associated modifications in proteins content, evaluated through DNA and proteins synthesis, at environmentally relevant dose of dioxins, brought by a typical environmental PCDD/F mixture. The response is time and species dependent. After completion of the identification of proteins affected by the intoxication, we will identify a set of specific proteins whose expression is correlated to the dioxin dose and submit the cell culture to the treatment with a single chemical as well as with mixtures. We hope that this will allow us to construct and validate a set of protein biomarkers of exposure to pollutants that will show a predictive aspect for unknown chemicals. The quantitative analysis of the set of biomarkers can then be a more specific bioassay and an alternative to physico-chemical or other already established bioanalytical methods for screening purposes. (C) 2004 Elsevier B.V. All rights reserved.
Disciplines :
Chemistry
Author, co-author :
Brenez, C.
Gerkens, P.
Mazzucchelli, Gabriel  ;  Université de Liège - ULiège > Centre interfac. d'analyse des résidus en traces (CART)
Jauniaux, Thierry ;  Université de Liège - ULiège > Département de morphologie et pathologie > Département de morphologie et pathologie
Eppe, Gauthier  ;  Université de Liège - ULiège > Centre interfac. d'analyse des résidus en traces (CART)
De Pauw, Edwin  ;  Université de Liège - ULiège > Chimie physique, spectrométrie de masse
De Pauw-Gillet, Marie-Claire ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
Language :
English
Title :
A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals
Publication date :
08 August 2004
Journal title :
Talanta
ISSN :
0039-9140
eISSN :
1873-3573
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
63
Issue :
5
Pages :
1225-1230
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 August 2009

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