ORBi: Brenez C. - A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals

Reference : A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on...


	Document type :	Scientific journals : Article
	Discipline(s) :	Physical, chemical, mathematical & earth Sciences : Chemistry
	To cite this reference:	http://hdl.handle.net/2268/18687

Title :	A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals
Language :	English
Author, co-author :	Brenez, C. [> > > >]
	Gerkens, P. [> > > >]
	Mazzucchelli, Gabriel [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
	Jauniaux, Thierry [Université de Liège - ULg > Département de morphologie et pathologie > Département de morphologie et pathologie >]
	Eppe, Gauthier [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
	De Pauw, Edwin [Université de Liège - ULg > > Chimie physique, spectrométrie de masse >]
	Gillet, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
Publication date :	8-Aug-2004
Journal title :	Talanta
Publisher :	Elsevier Science Bv
Volume :	63
Issue/season :	5
Pages :	1225-1230
Audience :	International
ISSN :	0039-9140
City :	Amsterdam
Keywords :	[en] PCDD/F mixture ; seal PBMC ; lymphoproliferation ; proteomics
Abstract :	[en] The cell lines chosen have demonstrated a positive response in terms of cell proliferation and associated modifications in proteins content, evaluated through DNA and proteins synthesis, at environmentally relevant dose of dioxins, brought by a typical environmental PCDD/F mixture. The response is time and species dependent. After completion of the identification of proteins affected by the intoxication, we will identify a set of specific proteins whose expression is correlated to the dioxin dose and submit the cell culture to the treatment with a single chemical as well as with mixtures. We hope that this will allow us to construct and validate a set of protein biomarkers of exposure to pollutants that will show a predictive aspect for unknown chemicals. The quantitative analysis of the set of biomarkers can then be a more specific bioassay and an alternative to physico-chemical or other already established bioanalytical methods for screening purposes. (C) 2004 Elsevier B.V. All rights reserved.
Permalink :	http://hdl.handle.net/2268/18687

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