Reference : A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on...
Scientific journals : Article
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/18687
A strategy to identify specific biomarkers related to the effects of a PCDD/F mixture on the immune system of marine mammals
English
Brenez, C. [> > > >]
Gerkens, P. [> > > >]
Mazzucchelli, Gabriel mailto [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
Jauniaux, Thierry mailto [Université de Liège - ULg > Département de morphologie et pathologie > Département de morphologie et pathologie >]
Eppe, Gauthier mailto [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
De Pauw, Edwin mailto [Université de Liège - ULg > > Chimie physique, spectrométrie de masse >]
Gillet, Marie-Claire mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
8-Aug-2004
Talanta
Elsevier Science Bv
63
5
1225-1230
Yes (verified by ORBi)
International
0039-9140
Amsterdam
[en] PCDD/F mixture ; seal PBMC ; lymphoproliferation ; proteomics
[en] The cell lines chosen have demonstrated a positive response in terms of cell proliferation and associated modifications in proteins content, evaluated through DNA and proteins synthesis, at environmentally relevant dose of dioxins, brought by a typical environmental PCDD/F mixture. The response is time and species dependent. After completion of the identification of proteins affected by the intoxication, we will identify a set of specific proteins whose expression is correlated to the dioxin dose and submit the cell culture to the treatment with a single chemical as well as with mixtures. We hope that this will allow us to construct and validate a set of protein biomarkers of exposure to pollutants that will show a predictive aspect for unknown chemicals. The quantitative analysis of the set of biomarkers can then be a more specific bioassay and an alternative to physico-chemical or other already established bioanalytical methods for screening purposes. (C) 2004 Elsevier B.V. All rights reserved.
http://hdl.handle.net/2268/18687

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