Article (Scientific journals)
Investigation of clostridium difficile interspecies relatedness using multilocus sequence typing, multilocus variable-number tandem-repeat analysis and antimicrobial susceptibility testing
Rodriguez Diaz, Cristina; Avesani, Véronique; Taminiau, Bernard et al.
2015In Veterinary Journal, 206, p. 349-355
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Keywords :
Animals; Humans; Clostridium difficile; Multilocus sequence typing; Multilocus variable-number tandem-repeat analysis
Abstract :
[en] Multilocus sequence typing (MLST), multilocus variable-number tandem-repeat analysis (MLVA) and antimicrobial susceptibility were performed on 37 animal and human C. difficile isolates belonging to 15 different PCR-ribotypes in order to investigate the relatedness of human and animal isolates and to identify possible transmission routes. MLVA identified a total of 21 different types while MLST only distinguished 12 types. Identical C. difficile strains were detected in the same animal species for PCR-ribotypes 014, 078, UCL 16U and UCL 36, irrespective of their origin or the isolation date. Non clonal strains were found among different hosts; however, a high genetic association between pig and cattle isolates belonging to PCR-ribotype 078 was revealed. MLVA also showed genetic differences that clearly distinguished human from animal strains. For a given PCR-ribotype, human and animal strains presented a similar susceptibility to the antimicrobials tested. All strains were susceptible to vancomycin, metronidazole, chloramphenicol and rifampicin, while PCR-ribotypes 078, UCL 5a, UCL 36 and UCL 103 were associated with erythromycin resistance. The data suggest a wide dissemination of clones at hospitals and breeding-farms or a contamination at the slaughterhouse, but less probability of interspecies transmission. However, further highly discriminatory genotyping methods are necessary to elucidate interspecies and zoonotic transmission of C. difficile.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Rodriguez Diaz, Cristina ;  Université de Liège > Département de sciences des denrées alimentaires (DDA) > Technologie des denrées alimentaires
Avesani, Véronique;  Catholic University of Leuven > Microbiology Unit
Taminiau, Bernard  ;  Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Van Broeck, Johan;  Catholic University of Leuven > Microbiology Unit
Brévers, Bastien;  University of Liège > Département des denrées alimentaires > Microbiologie des denrées alimentaires
Delmée, Michel;  Catholic University of Leuven > Microbiology Unit
Daube, Georges  ;  Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Language :
English
Title :
Investigation of clostridium difficile interspecies relatedness using multilocus sequence typing, multilocus variable-number tandem-repeat analysis and antimicrobial susceptibility testing
Publication date :
2015
Journal title :
Veterinary Journal
ISSN :
1090-0233
eISSN :
1532-2971
Publisher :
Elsevier, London, United Kingdom
Volume :
206
Pages :
349-355
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 October 2015

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