Reference : Study of the cholesterol extraction capacity of β-cyclodextrin and its derivatives, r...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/18676
Study of the cholesterol extraction capacity of β-cyclodextrin and its derivatives, relationships with their effects on endothelial cell viability and on membrane models
English
Castagne, Delphine mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique et magistrale >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Delattre, Luc mailto [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
Evrard, Brigitte mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Nusgens, Betty mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Piel, Géraldine mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique et magistrale >]
2009
Journal of Inclusion Phenomena and Macrocyclic Chemistry
Springer Netherlands
63
3-4
225-231
Yes (verified by ORBi)
International
0923-0750
1573-1111
[en] Cyclodextrins ; Membrane models ; Viability ; Cytotoxicity ; Endothelial cells ; Cholesterol
[en] Endothelial cells (HUVEC) were treated with β-cyclodextrin and hydroxypropylated or methylated derivatives solutions in order to quantify their cholesterol extraction capacity. Non-toxic concentrations of cyclodextrins (CDs) were determined following methyl thiazol tetrazolium (MTT) assays, total protein measurements, morphological observations and trypan blue assays. The residual cholesterol content of cells was measured and the extraction power of CDs compared to results obtained by phase solubility diagrams. Cholesterol was extracted with a dose-response relationship, the lowest residual cholesterol content being obtained with β-CD at 10 mM. Low substituted derivatives (Crysmeb® and hydroxypropyl-β-CD) maintained liposomes integrity (as shown before), were the less cytotoxic and presented the lowest affinity for cholesterol contrary to methylated derivatives with degrees of substitution around 2.
Researchers ; Professionals
http://hdl.handle.net/2268/18676
10.1007/s10847-008-9510-9

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