ORBi: Castagne Delphine - Study of the cholesterol extraction capacity of β-cyclodextrin and its derivatives, relationships with their effects on endothelial cell viability and on membrane models

Reference : Study of the cholesterol extraction capacity of β-cyclodextrin and its derivatives, rela...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Pharmacy, pharmacology & toxicology Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/18676

Title :	Study of the cholesterol extraction capacity of β-cyclodextrin and its derivatives, relationships with their effects on endothelial cell viability and on membrane models
Language :	English
Author, co-author :	Castagne, Delphine [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique et magistrale >]
	Fillet, Marianne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Delattre, Luc [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
	Evrard, Brigitte [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
	Nusgens, Betty [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
	Piel, Géraldine [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique et magistrale >]
Publication date :	2009
Journal title :	Journal of Inclusion Phenomena and Macrocyclic Chemistry
Publisher :	Springer Netherlands
Volume :	63
Issue/season :	3-4
Pages :	225-231
Audience :	International
ISSN :	0923-0750
e-ISSN :	1573-1111
Keywords :	[en] Cyclodextrins ; Membrane models ; Viability ; Cytotoxicity ; Endothelial cells ; Cholesterol
Abstract :	[en] Endothelial cells (HUVEC) were treated with β-cyclodextrin and hydroxypropylated or methylated derivatives solutions in order to quantify their cholesterol extraction capacity. Non-toxic concentrations of cyclodextrins (CDs) were determined following methyl thiazol tetrazolium (MTT) assays, total protein measurements, morphological observations and trypan blue assays. The residual cholesterol content of cells was measured and the extraction power of CDs compared to results obtained by phase solubility diagrams. Cholesterol was extracted with a dose-response relationship, the lowest residual cholesterol content being obtained with β-CD at 10 mM. Low substituted derivatives (Crysmeb® and hydroxypropyl-β-CD) maintained liposomes integrity (as shown before), were the less cytotoxic and presented the lowest affinity for cholesterol contrary to methylated derivatives with degrees of substitution around 2.
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/2268/18676
DOI :	10.1007/s10847-008-9510-9

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