Article (Scientific journals)
Study of the relationship between lipid binding properties of cyclodextrins and their effect on the integrity of liposomes
Piel, Géraldine; Piette, Marie; Barillaro, Valery et al.
2007In International Journal of Pharmaceutics, 338 (1-2), p. 35-42
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Keywords :
liposome; cyclodextrin; cholesterol; calcein; membrane permeability
Abstract :
[en] It is well known that cyclodextrins are able to extract lipids constituting membranes, increasing their fluidity and permeability. This behaviour towards biological membranes is directly linked to the toxicological effects of methylated cyclodextrins. However, confusion is currently made in the literature between the different methylated cyclodextrin derivatives. Moreover, a new methylated cyclodextrin derivative recently occurred in the market. the Crysmeb (R). We wanted to compare and understand the effect of the most currently used cyclodextrins on a model membrane. We studied the influence of natural cyclodextrins (beta CD and gamma CD), methylated derivatives (2,6-dimethyl-beta CD (Dimeb), 2,3,6-trimethyl-beta CD (Trimeb) and randomly methylated-beta CD (Rameb), as well as the new derivative Crysmeb), hydroxypropylated derivatives (HP beta CD of different substitution degrees and HP gamma CD) and the sulfobutylated derivative (SBE beta CD) on the release of a fluorescent marker encapsulated in the inner cavity of liposomes. It was shown that the observed effect on calcein release can be directly related to the affinity of cyclodextrins for both lipid components of liposomes, cholesterol and phosphatidylcholine. From this relationship, we were able to determine, for each cyclodextrin, a theoretical concentration giving rise to 50% or 100% calcein release. This theoretical concentration was confirmed experimentally. We have also showed that cyclodextrins which provoke calcein release also induce large structure modifications of liposomes. (c) 2007 Elsevier B.V. All rights reserved.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Piel, Géraldine ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique et magistrale
Piette, Marie ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Barillaro, Valery
Castagne, Delphine ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique et magistrale
Evrard, Brigitte  ;  Université de Liège - ULiège > Département de pharmacie > Pharmacie galénique
Delattre, Luc ;  Université de Liège - ULiège > Département de pharmacie > Département de pharmacie
Language :
English
Title :
Study of the relationship between lipid binding properties of cyclodextrins and their effect on the integrity of liposomes
Publication date :
29 June 2007
Journal title :
International Journal of Pharmaceutics
ISSN :
0378-5173
eISSN :
1873-3476
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
338
Issue :
1-2
Pages :
35-42
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 August 2009

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