Reference : Study of the relationship between lipid binding properties of cyclodextrins and their ef...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/18671
Study of the relationship between lipid binding properties of cyclodextrins and their effect on the integrity of liposomes
English
Piel, Géraldine mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique et magistrale >]
Piette, Marie mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Barillaro, Valery [> > > >]
Castagne, Delphine mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique et magistrale >]
Evrard, Brigitte mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Delattre, Luc mailto [Université de Liège - ULg > Département de pharmacie > Département de pharmacie >]
29-Jun-2007
International Journal of Pharmaceutics
Elsevier Science Bv
338
1-2
35-42
Yes (verified by ORBi)
International
0378-5173
Amsterdam
[en] liposome ; cyclodextrin ; cholesterol ; calcein ; membrane permeability
[en] It is well known that cyclodextrins are able to extract lipids constituting membranes, increasing their fluidity and permeability. This behaviour towards biological membranes is directly linked to the toxicological effects of methylated cyclodextrins. However, confusion is currently made in the literature between the different methylated cyclodextrin derivatives. Moreover, a new methylated cyclodextrin derivative recently occurred in the market. the Crysmeb (R). We wanted to compare and understand the effect of the most currently used cyclodextrins on a model membrane. We studied the influence of natural cyclodextrins (beta CD and gamma CD), methylated derivatives (2,6-dimethyl-beta CD (Dimeb), 2,3,6-trimethyl-beta CD (Trimeb) and randomly methylated-beta CD (Rameb), as well as the new derivative Crysmeb), hydroxypropylated derivatives (HP beta CD of different substitution degrees and HP gamma CD) and the sulfobutylated derivative (SBE beta CD) on the release of a fluorescent marker encapsulated in the inner cavity of liposomes. It was shown that the observed effect on calcein release can be directly related to the affinity of cyclodextrins for both lipid components of liposomes, cholesterol and phosphatidylcholine. From this relationship, we were able to determine, for each cyclodextrin, a theoretical concentration giving rise to 50% or 100% calcein release. This theoretical concentration was confirmed experimentally. We have also showed that cyclodextrins which provoke calcein release also induce large structure modifications of liposomes. (c) 2007 Elsevier B.V. All rights reserved.
Researchers ; Professionals
http://hdl.handle.net/2268/18671
10.1016/j.ijpharm.2007.01.015

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
article.pdfPublisher postprint644.44 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.