[en] Objectives To evaluate imaging changes occurring in a rat model of elastase-induced abdominal aortic aneurysm (AAA), with emphasis on the intraluminal thrombus (ILT) occurrence.
Methods The post-induction growth of the AAA diameter was characterized using ultrasound in 22 rats. ILT was reported on 13 rats that underwent 14 magnetic resonance imaging (MRI) 2-18 days post-surgery, and on 10 rats that underwent 18 fluoro-deoxyglucose (FDG) positron emission tomography (PET)/microcomputed tomography examinations 2-27 days post-surgery. Logistic regressions were used to establish the evolution with time of AAA length, diameter, ILT thickness, volume, stratification, MRI and FDG PET signalling properties, and histological assessment of inflammatory infiltrates.
Results All of the following significantly increased with time post-induction (p < 0.001): AAA length, AAA diameter, ILT maximal thickness, ILT volume, ILT iron content and related MRI signalling changes, quantitative uptake on FDG PET, and the magnitude of inflammatory infiltrates on histology. However, the aneurysm growth peak followed occurrence of ILT approximately 6 days after elastase infusion.
Conclusion Our model emphasizes that occurrence of ILT precedes AAA peak growth. Aneurysm growth is associated with increasing levels of iron, signalling properties changes in
both MRI and FDG PET, relating to its biological activities.
Research center :
GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège Giga-cardiovascular diseases
Disciplines :
Radiology, nuclear medicine & imaging
Author, co-author :
NCHIMI LONGANG, Alain ; Centre Hospitalier Universitaire de Liège - CHU > Service médical de radiodiagnostic
Courtois, Audrey ; Université de Liège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
EL HACHEMI, Mounia ; Centre Hospitalier Universitaire de Liège - CHU > Service médical de radiodiagnostic
Touat, Ziad
Drion, Pierre ; Université de Liège > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim.
WITHOFS, Nadia ; Centre Hospitalier Universitaire de Liège - CHU > Service médical de médecine nucléaire et imagerie onco
Warnock, Geoff
Bahri, Mohamed Ali ; Université de Liège > Centre de recherches du cyclotron
Dogné, Jean-Michel
CHERAMY-BIEN, Jean-Paul ; Centre Hospitalier Universitaire de Liège - CHU > Service de Chirurgie cardio-vasculaire et thoracique
SCHOYSMAN, Laurent ; Centre Hospitalier Universitaire de Liège - CHU > Service médical de radiodiagnostic
Joskin, Julien
Michel, Jean-Baptiste
Defraigne, Jean-Olivier ; Université de Liège > Département des sciences cliniques > Chirurgie cardio-vasculaire et thoracique
Plenevaux, Alain ; Université de Liège > Centre de recherches du cyclotron
SAKALIHASAN, Natzi ; Centre Hospitalier Universitaire de Liège - CHU > Service de Chirurgie cardio-vasculaire et thoracique
Dobrin, P.B., Pathophysiology and pathogenesis of aortic aneurysms. Current concepts (1989) Surg Clin N Am, 69, pp. 687-703. , COI: 1:STN:280:DyaL1MzhslOmug%3D%3D, PID: 2665139
Michel, J.B., Martin-Ventura, J.L., Egido, J., Novel aspects of the pathogenesis of aneurysms of the abdominal aorta in humans (2011) Cardiovasc Res, 90, pp. 18-27. , COI: 1:CAS:528:DC%2BC3MXjsFygsbc%3D, PID: 21037321
Sakalihasan, N., Limet, R., Defawe, O.D., Abdominal aortic aneurysm (2005) Lancet, 365, pp. 1577-1589. , COI: 1:STN:280:DC%2BD2M3jtlWksA%3D%3D, PID: 15866312
Touat, Z., Lepage, L., Ollivier, V., Dilation-dependent activation of platelets and prothrombin in human thoracic ascending aortic aneurysm (2008) Arterioscler Thromb Vasc Biol, 28, pp. 940-946. , COI: 1:CAS:528:DC%2BD1cXktlylsrg%3D, PID: 18292393
Sarda-Mantel, L., Coutard, M., Rouzet, F., 99mTc-annexin-V functional imaging of luminal thrombus activity in abdominal aortic aneurysms (2006) Arterioscler Thromb Vasc Biol, 26, pp. 2153-2159. , COI: 1:CAS:528:DC%2BD28XotVWlur4%3D, PID: 16857952
Fontaine, V., Touat, Z., el Mtairag, M., Role of leukocyte elastase in preventing cellular re-colonization of the mural thrombus (2004) Am J Pathol, 164, pp. 2077-2087. , COI: 1:CAS:528:DC%2BD2cXltFymsL0%3D, PID: 15161642
Houard, X., Rouzet, F., Touat, Z., Topology of the fibrinolytic system within the mural thrombus of human abdominal aortic aneurysms (2007) J Pathol, 212, pp. 20-28. , COI: 1:CAS:528:DC%2BD2sXmt1eisLw%3D, PID: 17352452
Martinez-Pinna, R., Madrigal-Matute, J., Tarin, C., Proteomic analysis of intraluminal thrombus highlights complement activation in human abdominal aortic aneurysms (2013) Arterioscler Thromb Vasc Biol, 33, pp. 2013-2020. , COI: 1:CAS:528:DC%2BC3sXhtFams7%2FJ, PID: 23702661
Martinez-Pinna, R., Lindholt, J.S., Madrigal-Matute, J., From tissue iron retention to low systemic haemoglobin levels, new pathophysiological biomarkers of human abdominal aortic aneurysm (2014) Thromb Haemost, 112, pp. 87-95. , COI: 1:CAS:528:DC%2BC2cXhslGgt77K, PID: 24599423
Defawe, O.D., Colige, A., Lambert, C.A., TIMP-2 and PAI-1 mRNA levels are lower in aneurysmal as compared to athero-occlusive abdominal aortas (2003) Cardiovasc Res, 60, pp. 205-213. , COI: 1:CAS:528:DC%2BD3sXns12rs7Y%3D, PID: 14522424
Fontaine, V., Jacob, M.P., Houard, X., Involvement of the mural thrombus as a site of protease release and activation in human aortic aneurysms (2002) Am J Pathol, 161, pp. 1701-1710. , COI: 1:CAS:528:DC%2BD38XptV2gtL4%3D, PID: 12414517
Folkesson, M., Kazi, M., Zhu, C., Presence of NGAL/MMP-9 complexes in human abdominal aortic aneurysms (2007) Thromb Haemost, 98, pp. 427-433. , COI: 1:CAS:528:DC%2BD2sXhtVeltr7O, PID: 17721627
Marbacher, S., Marjamaa, J., Bradacova, K., Loss of mural cells leads to wall degeneration, aneurysm growth, and eventual rupture in a rat aneurysm model (2014) Stroke, 45, pp. 248-254. , PID: 24222045
Mitchell, D.G., Burk, D.L., Jr., Vinitski, S., Rifkin, M.D., The biophysical basis of tissue contrast in extracranial MR imaging (1987) AJR Am J Roentgenol, 149, pp. 831-837. , COI: 1:STN:280:DyaL2szit1ylsg%3D%3D, PID: 3307357
Winner, M.W., 3rd, Sharkey-Toppen, T., Zhang, X., Iron and noncontrast magnetic resonance T2* as a marker of intraplaque iron in human atherosclerosis (2014) J Vasc Surg, , PID: 24674272
Lindholt, J.S., Bjorck, M., Michel, J.B., Anti-platelet treatment of middle-sized abdominal aortic aneurysms (2013) Curr Vasc Pharmacol, 11, pp. 305-313. , COI: 1:CAS:528:DC%2BC3sXpslKgsbk%3D, PID: 22724484
Touat, Z., Ollivier, V., Dai, J., Renewal of mural thrombus releases plasma markers and is involved in aortic abdominal aneurysm evolution (2006) Am J Pathol, 168, pp. 1022-1030. , COI: 1:CAS:528:DC%2BD28XislGgurY%3D, PID: 16507915
Anidjar, S., Salzmann, J.L., Gentric, D., Lagneau, P., Camilleri, J.P., Michel, J.B., Elastase-induced experimental aneurysms in rats (1990) Circulation, 82, pp. 973-981. , COI: 1:CAS:528:DyaK3MXhsVSrsr4%3D, PID: 2144219
Feldkamp, L.D.L., Kress, J., Practical cone-beam algorithm (1984) JOSA A, 1, pp. 612-619
Bahri, M.A., Plenevaux, A., Warnock, G., Luxen, A., Seret, A., NEMA NU4-2008 image quality performance report for the microPET focus 120 and for various transmission and reconstruction methods (2009) J Nucl Med, 50, pp. 1730-1738. , PID: 19759103
Defrise, M., Kinahan, P.E., Townsend, D.W., Michel, C., Sibomana, M., Newport, D.F., Exact and approximate rebinning algorithms for 3-D PET data (1997) IEEE Trans Med Imaging, 16, pp. 145-158. , COI: 1:STN:280:DyaK2s3lsF2qsQ%3D%3D, PID: 9101324
Nguyen-Legros, J., Bizot, J., Bolesse, M., Pulicani, J.P., "Diaminobenzidine black" as a new histochemical demonstration of exogenous iron (author's transl) (1980) Histochemistry, 66, pp. 239-244. , COI: 1:CAS:528:DyaL3cXkvFWnsrc%3D, PID: 7399970
Coutard, M., Touat, Z., Houard, X., Leclercq, A., Michel, J.B., Thrombus versus wall biological activities in experimental aortic aneurysms (2010) J Vasc Res, 47, pp. 355-366. , PID: 20016209
Defawe, O.D., Hustinx, R., Defraigne, J.O., Limet, R., Sakalihasan, N., Distribution of F-18 fluorodeoxyglucose (F-18 FDG) in abdominal aortic aneurysm: high accumulation in macrophages seen on PET imaging and immunohistology (2005) Clin Nucl Med, 30, pp. 340-341. , PID: 15827408
Reeps, C., Bundschuh, R.A., Pellisek, J., Quantitative assessment of glucose metabolism in the vessel wall of abdominal aortic aneurysms: correlation with histology and role of partial volume correction (2013) Int J Cardiovasc Imaging, 29, pp. 505-512. , PID: 22772434
Nchimi, A., Cheramy-Bien, J.P., Gasser, T.C., Multifactorial relationship between 18F-fluoro-deoxy-glucose positron emission tomography signaling and biomechanical properties in unruptured aortic aneurysms (2014) Circ Cardiovasc Imaging, 7, pp. 82-91. , PID: 24190906
English, S.J., Piert, M.R., Diaz, J.A., Increased 18F-FDG uptake is predictive of rupture in a novel rat abdominal aortic aneurysm rupture model (2015) Ann Surg, 261, pp. 395-404. , PID: 24651130
Michineau, S., Dai, J., Gervais, M., Aortic length changes during abdominal aortic aneurysm formation, expansion and stabilisation in a rat model (2010) Eur J Vasc Endovasc Surg, 40, pp. 468-474. , COI: 1:STN:280:DC%2BC3cbjtVKrtw%3D%3D, PID: 20554458
Rai, D., Wisniowski, B., Bradshaw, B., Abdominal aortic aneurysm calcification and thrombus volume are not associated with outcome following endovascular abdominal aortic aneurysm repair (2014) Eur Radiol, 24, pp. 1768-1776. , PID: 24817004
Nchimi, A., Defawe, O., Brisbois, D., MR Imaging of Iron Phagocytosis in Intraluminal Thrombi of Abdominal Aortic Aneurysms in Humans (2010) Radiology, 254, pp. 973-981. , PID: 20177108
Richards, J.M., Semple, S.I., MacGillivray, T.J., Abdominal aortic aneurysm growth predicted by uptake of ultrasmall superparamagnetic particles of iron oxide: a pilot study (2011) Circ Cardiovasc Imaging, 4, pp. 274-281. , PID: 21304070
Klink, A., Heynens, J., Herranz, B., In vivo characterization of a new abdominal aortic aneurysm mouse model with conventional and molecular magnetic resonance imaging (2011) J Am Coll Cardiol, 58, pp. 2522-2530. , PID: 22133853
Rouzet, F., Bachelet-Violette, L., Alsac, J.M., Radiolabeled fucoidan as a p-selectin targeting agent for in vivo imaging of platelet-rich thrombus and endothelial activation (2011) J Nucl Med, 52, pp. 1433-1440. , COI: 1:CAS:528:DC%2BC3MXht1yisrrK, PID: 21849401
Nahrendorf, M., Keliher, E., Marinelli, B., Detection of macrophages in aortic aneurysms by nanoparticle positron emission tomography-computed tomography (2011) Arterioscler Thromb Vasc Biol, 31, pp. 750-757. , COI: 1:CAS:528:DC%2BC3MXjt1Gnurc%3D, PID: 21252070
de Valk, B., Marx, J.J., Iron, atherosclerosis, and ischemic heart disease (1999) Arch Intern Med, 159, pp. 1542-1548. , PID: 10421276
Nguyen, V.L., Leiner, T., Hellenthal, F.A., Abdominal Aortic Aneurysms with High Thrombus Signal Intensity on Magnetic Resonance Imaging are Associated with High Growth Rate (2014) Eur J Vasc Endovasc Surg
Morin-Roy, F., Kauffmann, C., Tang, A., Impact of contrast injection and stent-graft implantation on reproducibility of volume measurements in semiautomated segmentation of abdominal aortic aneurysm on computed tomography (2014) Eur Radiol, 24, pp. 1594-1601. , PID: 24801978
