Poster (Scientific congresses and symposiums)
Orbivirus screening on dried blood spots from captive oryx in United Arab Emirates stresses the importance of pre-import measures
Martinelle, Ludovic; Haegeman, Andy; Lignereux, Louis et al.
2015Xth International Congress of Veterinary Virology, ESVV 2015, combined with the 9th annual EPIZONE meeting
 

Files


Full Text
EPIZONE2015LMe.pdf
Publisher postprint (1.72 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
bluetongue; orbivirus; oryx
Abstract :
[en] Objective: Following reintroduction and conservation programs of the Arabian oryx (Oryx leucoryx) and the scimitar horned oryx (SHO, Oryx dammah) in the United Arab Emirates (UAE), import of animals from wild game ranches in the United States of America (USA) is not uncommon. Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are orbiviruses that are the causative agents of bluetongue disease (BT) and epizootic hemorrhagic disease (EHD), respectively. BTV and EHDV are endemic in the UAE and the USA. Sheep and some wild ruminant species are usually severely affected by BT whereas EHD mostly affects wild animals and sometimes cattle. The objective of this study was to estimate the prevalence of these orbiviruses in Arabian and SHO from captive herds in the UAE using serology and molecular virology. Dry blood spot sampling for orbivirus screening is also discussed. Methods: A total of 175 SHO and 16 Arabian oryx were sampled. The latters were imported from Texas (USA) two weeks before sampling. All sampled animals belonged to captive herds spread over the Al Wathba area. For biosecurity reasons and to simplify blood storage, elutes from dried blood spot were used for serological and virological tests. Drops of about 80 µl of blood were dispensed on Whatman protein saver cards, and then allowed to dry in the dark at room temperature for 48 hours. Blood spots were punched out in paper discs with a 6 mm diameter punch and diluted in 250 µl PBS and Tween 20 0.05%. Eluted samples were incubated overnight at room temperature and then used immediately or stored at -80°C. To assess the most suitable ELISA kit to detect anti-BTV antibodies from the oryx discs, similar discs were prepared using blood issued from BTV seropositive and viremic as well as seronegative and non-viremic cattle. Elutes from discs with dried-blood from cattle were tested by BTV competitive ELISA (cELISA), sandwich ELISA (sELISA) and indirect ELISA (iELISA) and compared to cELISA performed directly on the serum of the same animals. iELISA on cattle paper discs gave the best correspondence with cELISA on cattle serum and was therefore used to test the oryx paper discs. Subsequently oryx paper discs were tested to detect antibodies against EHDV by cELISA. All the paper discs elutes from Arabian oryx and ELISA positive elutes from SHO were also tested by pan-BTV RTqPCR targeting a fragment of BTV segment 5 and detecting all BTV serotypes. Serotype specific end-point RT-PCR targeting a fragment of segment 2 of BTV2, BTV8, BTV10, BTV11, BTV13 and BTV17 were performed on pan-BTV positive samples. Results: Three out of 175 SHO and eight out of 16 Arabian oryx were found BTV seropositive by iELISA. None of the animals could be found seropositive against EHDV. BTV genome was detected in 1/3 seropositive SHO and in 5/16 of the Arabian oryx, amongst those 2/5 were seronegative. Overall Cq values were high (33-39). End point PCR failed to detect positive samples for any of the tested serotypes. Conclusion: BTV seroprevalence and RNA detection in SHO was very limited. By contrast BTV could be demonstrated in 5/16 imported Arabian oryx by molecular virology and in 8/16 by serology. The sampling was realized two weeks after the animals arrived in UAE and some oryx were viremic and seronegative, possibly suggesting a recent infection. Among the local SHO a low BTV seroprevalence was observed (3/175) and no animals were found positive to EHDV. This result was quite surprising because previous studies showed a higher BTV seroprevalence in domestic and wild ruminants of the Arabian Peninsula with wide local variations. In addition, dried blood spot testing has been demonstrated being a convenient and reliable method of sampling when storage conditions are hazardous. BTV serotypes could not be determined by end-point RT-PCR. At least 15 different BTV serotypes were reported in the USA and at least 10 in the Middle East, thus the oryx could be infected by a serotype not tested so far. Since RTqPCR positive values were high, the sensitivity of end-point RT-PCR might be insufficient to detect BTV out of eluted blood spots. Additional testing will be performed to identify the virus on the serotype level and therefore provide new insights to clarify the origin of the infection of the oryx. These results stress the need for pre-import risk assessment, precaution and implementation of biosecurity measures when considering translocation of wild ruminant species susceptible to BTV and EHDV.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Martinelle, Ludovic ;  Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.
Haegeman, Andy;  Centre d'Etudes et de Recherches Vétérinaires Agronomiques = Centrum voor Onderzoek in Diergeneeskunde en Agrochemie - CERVA=CODA
Lignereux, Louis ;  Environment Agency - Abu Dhabi
Chaber, Anne-Lise ;  Wildlife Consultant L.L.C - United Arab Emirates
Dal Pozzo, Fabiana ;  Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.
De Leeuw, Ilse;  Centre d'Etudes et de Recherches Vétérinaires Agronomiques = Centrum voor Onderzoek in Diergeneeskunde en Agrochemie - CERVA=CODA
De Clercq, Kris;  Centre d'Etudes et de Recherches Vétérinaires Agronomiques = Centrum voor Onderzoek in Diergeneeskunde en Agrochemie - CERVA=CODA
Saegerman, Claude  ;  Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appl. aux sc. vétér.
Language :
English
Title :
Orbivirus screening on dried blood spots from captive oryx in United Arab Emirates stresses the importance of pre-import measures
Publication date :
01 September 2015
Event name :
Xth International Congress of Veterinary Virology, ESVV 2015, combined with the 9th annual EPIZONE meeting
Event date :
31 aout au 3 septembre 2015, Montpellier, France
Available on ORBi :
since 17 September 2015

Statistics


Number of views
125 (8 by ULiège)
Number of downloads
163 (8 by ULiège)

Bibliography


Similar publications



Contact ORBi