Poster (Scientific congresses and symposiums)
Small Heat Shock Proteins (sHSPs), specific markers of exercise-induced muscle damage
Hody, Stéphanie; Simonet, Arnaud; Lacrosse, Zoé et al.
2015Cell symposia : Exercise metabolism
 

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Keywords :
Eccentric contraction; Exercise-induced muscle damage; Heat Shock Proteins; Muscle training; Exercise; Delayed-Onset Muscle Soreness; Proteomics; Immunofluorescence; Contraction excentrique; Microlésions musculaires; Protéines de stress; Entraînement; Courbatures; Douleurs musculaires d'apparition retardée; Protéomique
Abstract :
[en] Unaccustomed eccentric (ECC) exercise may cause exercise-induced muscle damage (EIMD) whereas repeating submaximal ECC sessions is known to prevent this damage. The present study aimed to better understand the molecular mechanisms underlying EIMD and subsequent adaptation. C57BL adult mice were submitted to different treadmill running protocols: IEE (intense ECC exercise), ET (ECC training), CT (concentric training), LT (level training), ET+IEE, CT+IEE. A 2D-DIGE proteomic analysis on mouse quadriceps muscles revealed that HSP25 and alpha-crystallin B chain were significantly more abundant in IEE, CT+IEE, ET+IEE and ET groups compared to unexercised mice. Interestingly, immunofluorescence analysis highlighted that all damaged fibers also showed positive staining for sHSPs. In contrast, sHSPs+ fibers are not necessarily damaged, suggesting that the sHSPs response precedes increased sarcolemma permeability. Our data demonstrate that ECC exercise specifically increases sHSPs expression which may represent an early marker of damage and/or adaptation of the muscle fibers to ECC contraction.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Hody, Stéphanie ;  Université de Liège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Simonet, Arnaud ;  Université de Liège - ULiège > Master kiné. & réadapt.
Lacrosse, Zoé ;  Université de Liège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Leprince, Pierre ;  Université de Liège > GIGA - Neurosciences
Croisier, Jean-Louis ;  Université de Liège > Département des sciences de la motricité > Kinésithérapie générale et réadaptation
Language :
English
Title :
Small Heat Shock Proteins (sHSPs), specific markers of exercise-induced muscle damage
Publication date :
12 July 2015
Number of pages :
Poster A0
Event name :
Cell symposia : Exercise metabolism
Event organizer :
Cell
Event place :
Amsterdam, Netherlands
Event date :
du 12 juillet 2015 au 14 juillet 2015
Audience :
International
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
ULiège - Université de Liège [BE]
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since 14 September 2015

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