[en] The formation of the cuticle was investigated during moulting of the isopod crustacean Ligia italica. The intermoult cuticle is a four-layered lamellar structure composed of chitin-protein fibrils and mineralized in its upper half. The distribution of calcium carbonate in cuticle during moult cycle was determined by cytochemical methods and X-ray microanalysis, Epi-and exocuticle are secreted during premoult. Calcium is resorbed from the old cuticle and accumulates in the ecdysal gap as calcium granules. Endocuticle is secreted after moult when the mineralization of exocuticle starts. The shape and ultrastructure of epithelial cells change during cuticle secretion and mineralization. Mitochondria, bundles of filaments, calcium granules and large amounts of glycogen accumulate in the apical cytoplasm of cells in premoult animals.
Disciplines :
Zoology
Author, co-author :
Strus, Jasna; University of Ljubljana, Slovenia > Biology
Compère, Philippe ; Université de Liège > Département de Biologie, Ecologie et Evolution > Département de Biologie, Ecologie et Evolution
Language :
English
Title :
Ultrastructural analysis of the integument during the moult cycle in Ligia italica (Crustacea, Isopoda)
Price, J.B., Holdich, D.M., An Ultrastructural Study of the Integument during the Moult Cycle of the Woodlouse, Oniscus asellus (Crustacea, Isopoda) (1980) Zoomorphologie, 95 (3), pp. 250-263
Steel, C.G.H., Storage and translocation of integumentary calcium during the moult cycle of the terrestrial isopod Oniscus asellus (L.) (1993) Can J Zool, 71, pp. 4-10
Wood, S., Russell, J.D., On the nature of the calcium carbonate in the exoskeleton of the woodlouse Oniscus asellus L. (Isopoda, Oniscoidea) (1987) Crustaceana, 53 (1), pp. 49-53
Ziegler, A., Ultrastructure and Electron Spectroscopic Diffraction Analysis of the Sternal Calcium Deposits of Porcellio scaber Latr. (Isopoda, Crustacea) (1994) J Struct Biol, 112, pp. 110-116
Carasso, N., Favard, P., Mise en évidence du calcium dans les myonemes pédonuclaires de ciliés péritriches (1966) J. Microscopy, 5, pp. 759-770
Karnovsky, M.J., Use of ferrocyanide-reduced osmium tetroxide in electron microscopy (1971) J Cell Biol, 51, pp. 146-A
Meyran, J.C., Graf, F., Nicaise, G., Calcium pathway through a mineralizing epithelium in the crustacean Orchestia in premoult: Ultrastructural cytochemistry and X-ray microanalysis (1984) Tissue & Cell, 18, pp. 267-283