Article (Scientific journals)
Quantification of hypoglycin A in serum using aTRAQ((R)) assay
BOEMER, François; DEBERG, Michelle; SCHOOS, Roland et al.
2015In Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 997, p. 75-80
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Keywords :
Amino acids; Atypical myopathy; Hypoglycin A; Mass spectrometry; aTRAQ
Abstract :
[en] BACKGROUND: Hypoglycin A has been recently identified has the causal agent of atypical myopathy (AM) in horses. Its identification and quantification in equine's biological fluids is thus a major concern to confirm maple poisoning and to provide insight into the poorly understood mechanism of hypoglycin A intoxication. METHODS: Quantification of hypoglycin A has been achieved with the aTRAQ kit for amino acid analysis of physiological fluids (AB Sciex). Acquisition method on mass spectrometer has been updated to record the hypoglycin A specific MRM transition. RESULTS: Outlined accuracy profiles demonstrated very reliable data. A good linearity was observed from 0.09 to 50mumol/L and precision was very good with coefficient of variation below 8%. Fifty-five samples collected from 25 confirmed AM horses revealed significant hypoglycin A concentrations, while toxin was not found in serum of 8 control animals. CONCLUSIONS: The described aTRAQ variant method has been analytically and clinically validated. The reliability of our approach is thus demonstrated into the workup of atypical myopathy.
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Genetics & genetic processes
Author, co-author :
BOEMER, François  ;  Centre Hospitalier Universitaire de Liège - CHU > Génétique
DEBERG, Michelle ;  Centre Hospitalier Universitaire de Liège - CHU > Génétique
SCHOOS, Roland ;  Centre Hospitalier Universitaire de Liège - CHU > Génétique
Baise, Etienne ;  Université de Liège > Département de productions animales > Biostatistique, économie, sélection animale
Amory, Hélène ;  Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Médecine interne des équidés
Gault, Gilbert
Carlier, Jeremy
Gaillard, Yvan
Marcillaud-Pitel, Christel
Votion, Dominique  ;  Université de Liège > Scientifiques attachés au Doyen (F MV)
Language :
English
Title :
Quantification of hypoglycin A in serum using aTRAQ((R)) assay
Publication date :
2015
Journal title :
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
ISSN :
1570-0232
eISSN :
1873-376X
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
997
Pages :
75-80
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 31 August 2015

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