ORBi: Servais Anne-Catherine - Optimization of the separation of beta-blockers by ion-pair capillary electrophoresis in non-aqueous media using univariate and multivariate approaches

Reference : Optimization of the separation of beta-blockers by ion-pair capillary electrophoresis in...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Pharmacy, pharmacology & toxicology
	To cite this reference:	http://hdl.handle.net/2268/18523

Title :	Optimization of the separation of beta-blockers by ion-pair capillary electrophoresis in non-aqueous media using univariate and multivariate approaches
Language :	English
Author, co-author :	Servais, Anne-Catherine [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Fillet, Marianne [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Chiap, Patrice [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
	Abushoffa, Adel M. [Université de Liège - ULG > Département de pharmacie > Analyse des médicaments > >]
	Hubert, Philippe [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments > >]
	Crommen, Jacques [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Publication date :	Dec-2002
Journal title :	Journal of Separation Science
Publisher :	Wiley-V C H Verlag Gmbh
Volume :	25
Issue/season :	15-17
Pages :	1087-1095
Audience :	International
ISSN :	1615-9314
City :	Weinheim
Keywords :	[en] non-aqueous capillary electrophoresis ; ion-pairing ; optimization ; beta-blockers
Abstract :	[en] The separation of four beta-blocking drug substances (atenolol, sotalol, betaxolol, and metoprolol) selected as model basic analytes has been investigated in non-aqueous capillary electrophoresis (NACE) using the principle of ion-pair formation. Camphorsulphonate was selected as the counter-ion in a background electrolyte (BGE) made up of formate buffer in a mixture of acetonitrile/methanol or acetonitrile/ethanol. The influence on resolution of the concentration of the counter-ion, the nature and proportion of the organic solvents, as well as the concentration of the ionic components of the BGE was first studied by a univariate approach. An experimental design was then applied to estimate possible quadratic effects and first-order interactions. To identify the most important factors affecting the separation of the four beta-blockers, a two-level fractional factorial design with 16 experimental points was applied as a screening test. A three factor Box-Behnken design with 12 experimental points was used to predict and optimize the selectivity. Finally the optimal conditions obtained by the univariate and the multivariate, approaches were compared.
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Permalink :	http://hdl.handle.net/2268/18523

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