Reference : Determination of flurbiprofen enantiomers in plasma using a single-isomer amino cyclo...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/18522
Determination of flurbiprofen enantiomers in plasma using a single-isomer amino cyclodextrin derivative in nonaqueous capillary electrophoresis.
English
Rousseau, Anne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Pedrini, Matteo [Université de Liège - ULG > Département de pharmacie > Analyse des médicaments > >]
Chiap, Patrice mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pharmacologie clinique >]
Ivanyi, Robert [Cyclolab > Cyclodextrin R&D > > >]
Crommen, Jacques mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Fillet, Marianne mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
Servais, Anne-Catherine mailto [Université de Liège - ULg > Département de pharmacie > Analyse des médicaments >]
2008
Electrophoresis
Vch Publishers
29
17
3641-8
Yes (verified by ORBi)
International
0173-0835
Weinheim
Germany
[en] Cationic cyclodextrin ; Flurbiprofen ; Nonaqueous capillary electrophoresis
[fr] Plasma samples ; Quantitative determination
[en] A nonaqueous capillary electrophoresis (NACE) assay was developed for the separation and determination of flurbiprofen enantiomers in plasma samples using 6-monodeoxy-6-mono(3-hydroxy)propylamino-beta-cyclodextrin as chiral selector. The nonaqueous background electrolyte was made up of 40 mM ammonium acetate in methanol (MeOH), and flufenamic acid was employed as internal standard. Solid-phase extraction was used for sample cleanup prior to the NACE separation.The NACE method reproducibility was optimized by evaluating different capillary washing sequences between runs. After having tested various conditions, trifluoroacetic acid (1 M) in MeOH was finally selected. Concerning the solid-phase extraction procedure, good and reproducible analyte recoveries were obtained using MeOH for protein denaturation and a polymeric phase combining hydrophobic interactions with anion exchange properties (Oasis) MAX) was selected as extraction sorbent. The method selectivity was not only demonstrated toward a blank plasma sample but also toward other non-steroidal anti-inflammatory drugs. The method was then successfully validated with respect to response function, trueness, precision, accuracy, linearity and limit of quantification.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds Léon Fredericq
http://hdl.handle.net/2268/18522
10.1002/elps.200700919

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