[en] BACKGROUND: Laccase is an intensively researched enzyme for industrial use. Except for
decolorisation measurements, HPLC analysis is the conventional method for monitoring the
phenolic removal during laccase enzyme reaction. This paper reports an investigation of the
continuous UV absorbance follow-up of the laccase reaction with steam pretreated poplar
hydrolysate.
RESULTS: Vanillin was used as a model substrate and lignocellulose xylose rich fraction
(XRF) as a biologically complex substrate for laccase detoxification. The reaction was
followed by HPLC-UV as well as by UV spectrometric measurements. Results suggest that
the reaction can be successfully monitored by measuring the change of UV absorbance at 280
nm, without previous compound separation. In case of XRF experiments the
spectrophotometric follow-up is especially useful, as HPLC analysis takes a long time and
provides less information than in case of single substrates. The method seems to be suitable
for optimization and process control.
CONCLUSION: The obtained results can help to construct a fast, easy and straightforward
monitoring system for laccase-phenolic substrate reactions.
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Sòti, Valentin; University of Antwerp - Faculty of Applied Engineering
Jacquet, Nicolas ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle
Apers, Sandra; University of Antwerp - Faculty of Pharmaceutical, Biomedical and Veterinary Sciences
Richel, Aurore ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle
Lenaerts, Silvia; University of Antwerp - DuEL, Sustainable Energy, air and water technology
Cornet, Iris; University of Antwerp - Faculty of Applied Engineering
Language :
English
Title :
Monitoring the laccase reaction of vanillin and poplar hydrolysate
Publication date :
2016
Journal title :
Journal of Chemical Technology and Biotechnology
ISSN :
0268-2575
Publisher :
John Wiley & Sons Ltd., Chichester, United Kingdom
Thurston CF, The structure and function of fungal laccases. Microbiology 140:19-26 (1994).
Kunamneni A, Plou FJ, Ballesteros A and Alcalde M, Laccases and their applications: a patent review. Recent Patent Biotechnol 2:10-24 (2008).
Durán N, Rosa MA, D'Annibale A and Gianfreda L, Applications of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review. Enzyme Microbiol Technol 31:907-931 (2002).
Busca G, Berardinelli S, Resini C and Arrighi L, Technologies for the removal of phenol from fluid streams: a short review of recent developments. J Hazard Mater 160:265-288 (2008). DOI:10.1016/j.jhazmat.2008.03.045
Madhavi V and Lele SS, Laccase: properties and applications. BioResources 4:1694-1717 (2009).
Fernández-Fernández M, Sanromán M and Moldes D, Recent developments and applications of immobilized laccase. Biotechnol Adv 31:1808-1825 (2013).
Duran N and Esposito E, Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review. Appl Catal B 28:83-99 (2000).
Peralta-Zamora P, Pereira CM, Tiburtius ER, Moraes SG, Rosa MA, Minussi RC and Durán N, Decolorization of reactive dyes by immobilized laccase. Appl Catal B 42:131-144 (2003). 5
Champagne PP and Ramsay JA, Reactive blue 19 decolouration by laccase immobilized on silica beads. Appl. Microbiol Biotechnol 77:819-823 (2007).
Piacquadio P, De Stefano G, Sammartino M and Sciancalepore V, Phenols removal from apple juice by laccase immobilized on Cu 2+-chelate regenerable carrier. Biotechnol Technol 11:515-517 (1997).
Lante A, Crapisi A, Pasini G, Zamorani A and Spettoli P, Immobilized laccase for must and wine processing. Ann N Y Acad Sci 672:558-562 (1992).
Alvira P, Moreno AD, Ibarra D, Sáez F and Ballesteros M, Improving the fermentation performance of saccharomyces cerevisiae by laccase during ethanol production from steam-exploded wheat straw at high-substrate loadings. Biotechnol Prog 29:74-82 (2013).
Jurado M, Prieto A, Martínez-Alcalá Á, Martínez ÁT and Martínez MJ, Laccase detoxification of steam-exploded wheat straw for second generation bioethanol. Bioresource Technol 100:6378-6384 (2009).
Jönsson LJ, Palmqvist E, Nilvebrant NO and Hahn-Hägerdal B, Detoxification of wood hydrolysates with laccase and peroxidase from the white-rot fungus Trametes versicolor. Appl Microbiol Biotechnol 49:691-697 (1998).
Ward G, Hadar Y and Dosoretz CG, Lignin peroxidase-catalyzed polymerization and detoxification of toxic halogenated phenols. J Chem Technol Biotechnol 78:1239-1245 (2003).
Steevensz A, Al-Ansari MM, Taylor KE, Bewtra JK and Biswas N, Oxidative coupling of various aromatic phenols and anilines in water using a laccase from Trametes villosa and insights into the 'PEG effect'. J Chem Technol Biotechnol 87:21-32 (2012).
Franciscon E, Piubeli F, Fantinatti-Garboggini F, Ragagnin de Menezes C, Serrano Silva I, Cavaco-Paulo A and Durrant LR, Polymerization study of the aromatic amines generated by the biodegradation of azo dyes using the laccase enzyme. Enzyme Microbiol Technol 46:360-365 (2010).
Desentis-Mendoza RM, Hernández-Sánchez H, Moreno A, Rojas del CE, Chel-Guerrero L, Tamariz J and Jaramillo-Flores ME, Enzymatic polymerization of phenolic compounds using laccase and tyrosinase from ustilago m aydis. Biomacromolecules 7:1845-1854 (2006).
Yu J, Taylor KE, Zou H, Biswas N and Bewtra JK, Phenol conversion and dimeric intermediates in horseradish peroxidase-catalyzed phenol removal from water. Environ Sci Technol 28:2154-2160 (1994).
Lahtinen M, Heinonen P, Oivanen M, Karhunen P, Kruus K and Sipilä J, On the factors affecting product distribution in laccase-catalyzed oxidation of a lignin model compound vanillyl alcohol: experimental and computational evaluation. Org Biomol Chem 11:5454-5464 (2013).
Fukuda T, Uchida H, Suzuki M, Miyamoto H, Morinaga H, Nawata H and Uwajima T, Transformation products of bisphenol A by a recombinant Trametes villosa laccase and their estrogenic activity. J Chem Technol Biotechnol 79:1212-1218 (2004).
Ganachaud C, Garfagnoli V, Tron T and Iacazio G, Trimerisation of indole through laccase catalysis. Tetrahedron Lett 49:2476-2478 (2008).
Shuttleworth KL and Bollag JM, Soluble and immobilized laccase as catalysts for the transformation of substituted phenols. Enzyme Microbiol Technol 8:171-177 (1986).
Salis A, Pisano M, Monduzzi M, Solinas V and Sanjust E, Laccase from Pleurotus sajor-caju on functionalised SBA-15 mesoporous silica: immobilisation and use for the oxidation of phenolic compounds. J Mol Catal B: Enzyme 58:175-180 (2009).
Kolb M, Sieber V, Amann M, Faulstich M and Schieder D, Removal of monomer delignification products by laccase from Trametes versicolor. Bioresource Technol 26:298-304 (2011).
Galliker P, Hommes G, Schlosser D, Corvini PFX and Shahgaldian P, Laccase-modified silica nanoparticles efficiently catalyze the transformation of phenolic compounds. J Colloid Interface Sci. 349:98-105 (2010).
Osma JF, Toca-Herrera JL and Rodríguez-Couto S, Transformation pathway of Remazol Brilliant Blue R by immobilised laccase. Bioresource Technol 101:8509-8514 (2010).
Nilvebrant NO, Reimann A, Larsson S and Jönsson LJ, Detoxification of lignocellulose hydrolysates with ion-exchange resins. Appl Biochem Biotechnol 91:1-3 (2001).
Johannes C and Majcherczyk A, Laccase activity tests and laccase inhibitors. J Biotechnol 78:193-199 (2000).
Manole A, Herea D, Chiriac H and Melnig V, Laccase activity determination. Cuza University Scientific Annals Lasi 4:17-24 (2008).
Cuyckens F and Claeys M, Mass spectrometry in the structural analysis of flavonoids. J Mass Spectrom 39:1-15 (2004).
Navarra C, Goodwin C, Burton S, Danieli B and Riva S, Laccase-mediated oxidation of phenolic derivatives. J Mol Catal B: Enzyme 65:52-57 (2010).
Rittstieg K, Suurnakki A, Suortti T, Kruus K, Guebitz G and Buchert J, Investigations on the laccase-catalyzed polymerization of lignin model compounds using size-exclusion HPLC. Enzyme Microbiol Technol 31:403-410 (2002).
Larsson S, Reimann A, Nilvebrant NO and Jönsson LJ, Comparison of different methods for the detoxification of lignocellulose hydrolyzates of spruce. Appl Biochem Biotechnol 77:91-103 (1999).