Article (Scientific journals)
Monitoring the laccase reaction of vanillin and poplar hydrolysate
Sòti, Valentin; Jacquet, Nicolas; Apers, Sandra et al.
2016In Journal of Chemical Technology and Biotechnology, 91 (6), p. 1914-1922
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Keywords :
lignocellulose; steam explosion; laccase; poplar; vanillin
Abstract :
[en] BACKGROUND: Laccase is an intensively researched enzyme for industrial use. Except for decolorisation measurements, HPLC analysis is the conventional method for monitoring the phenolic removal during laccase enzyme reaction. This paper reports an investigation of the continuous UV absorbance follow-up of the laccase reaction with steam pretreated poplar hydrolysate. RESULTS: Vanillin was used as a model substrate and lignocellulose xylose rich fraction (XRF) as a biologically complex substrate for laccase detoxification. The reaction was followed by HPLC-UV as well as by UV spectrometric measurements. Results suggest that the reaction can be successfully monitored by measuring the change of UV absorbance at 280 nm, without previous compound separation. In case of XRF experiments the spectrophotometric follow-up is especially useful, as HPLC analysis takes a long time and provides less information than in case of single substrates. The method seems to be suitable for optimization and process control. CONCLUSION: The obtained results can help to construct a fast, easy and straightforward monitoring system for laccase-phenolic substrate reactions.
Disciplines :
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Author, co-author :
Sòti, Valentin;  University of Antwerp - Faculty of Applied Engineering
Jacquet, Nicolas  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle
Apers, Sandra;  University of Antwerp - Faculty of Pharmaceutical, Biomedical and Veterinary Sciences
Richel, Aurore  ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie biologique industrielle
Lenaerts, Silvia;  University of Antwerp - DuEL, Sustainable Energy, air and water technology
Cornet, Iris;  University of Antwerp - Faculty of Applied Engineering
Language :
English
Title :
Monitoring the laccase reaction of vanillin and poplar hydrolysate
Publication date :
2016
Journal title :
Journal of Chemical Technology and Biotechnology
ISSN :
0268-2575
Publisher :
John Wiley & Sons Ltd., Chichester, United Kingdom
Volume :
91
Issue :
6
Pages :
1914-1922
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 21 August 2015

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