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Article (Scientific journals)
Role of Nuclear Factor-Kappa B in Colon Cancer Cell Apoptosis Mediated by Aminopyropheophorbide Photosensitization
Matroule, J. Y.; Hellin, Anne-Cécile; Morliere, P. et al.
1999In Photochemistry and Photobiology, 70 (4), p. 540-8
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Abstract :
[en] Aminopyropheophorbide (APP) is a second generation of photosensitizer for photodynamic therapy (PDT). We demonstrated that APP strongly absorbed red light and, after being taken up by colon cancer cells (HCT-116 cells), was localized in cytoplasmic and internal membranes but not in mitochondria. The APP-mediated photosensitization was cytotoxic for HCT-116 cells through an induction of apoptosis. Indeed, DNA fragmentation (DNA laddering and terminal deoxyuridine nick-end labeling) and chromatin condensation (4',6-diamidine-2'-phenylindole staining) could be visualized soon after photosensitization. Because nuclear factor (NF)-kappa B is involved in the response to many photosensitizers, we also demonstrated its nuclear translocation in two waves: a rapid and transient one, followed by a slow and sustained phase. The NF-kappa B turned out to be involved in an antiapoptotic response to APP-mediated photosensitization because the HCT-116 cell line expressing the dominant negative mutant of inhibitor-kappa B alpha was more sensitive to apoptosis as measured by DNA fragmentation and caspase activation. These data unambiguously show that a membrane-located photosensitizer can lead to effective apoptosis, reinforcing the idea that PDT can be an effective means to eradicate colon cancer cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Matroule, J. Y.
Hellin, Anne-Cécile 
Morliere, P.
Fabiano, A. S.
Santus, R.
Merville, Marie-Paule ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Language :
English
Title :
Role of Nuclear Factor-Kappa B in Colon Cancer Cell Apoptosis Mediated by Aminopyropheophorbide Photosensitization
Publication date :
October 1999
Journal title :
Photochemistry and Photobiology
ISSN :
0031-8655
Publisher :
Wiley-Blackwell, United States - Kansas
Volume :
70
Issue :
4
Pages :
540-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 11 August 2009

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